MDCK Cell Line canine

84121903, canine kidney, Epithelial

• 別名: MDCK (NBL-2) Cells, Madin Darby Canine Kidney Cells, NBL-2 Cells
• UNSPSCコード: 41106514
• eCl@ss: 32190102

特性

• 製品名: MDCK Cell Line canine, 84121903
• 由来生物: canine kidney
• フォーム: cell suspension
• 成長モード: Adherent
• 核型: 2n = 78
• 形態: Epithelial
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Canine Cocker Spaniel kidney

細胞株の説明

From kidney of normal female adult Cocker Spaniel in 1958 by SH Madin and NB Darby (Madin Darby Canine Kidney). Supports growth of wide range of animal viruses: VSV (Indiana strain), infectious Canine Hepatitis, Vaccinia, Coxsackie B5, Adeno and reo viruses, SVEV.

アプリケーション

Virus studies: SVEV, VSV, Vaccinia, adeno and reoviruses, Coxsackie B5 et al

培地

EMEM（EBSS） + 2 mMグルタミン + 1％非必須アミノ酸（NEAA） + 10％ウシ胎児血清（FBS）

継代と培養方法

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Cells attach firmly and require at least 2 PBS washes prior to addition of trypsin/EDTA.

その他情報

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