VERO Cell Line

from African green monkey kidney, 84113001, A fibroblast-like kidney cell, non-WHO origin, Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES)

• 別名: Vero 81 Cells, VeroCCL81 Cells
• UNSPSCコード: 41106514

特性

• 由来生物: African green monkey kidney
• 成長モード: Adherent
• 核型: 2n = 60, modal no. 58
• 形態: Fibroblast-like
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

詳細

Vero cells are anchorage-dependent cells and have wide applications in molecular and cell biology research. Vero cells have a pseudo-diploid karyotype and are preferred cell lines for the propagation of various viruses, including poliovirus, rotavirus influenza and rabies virus. These cells are useful hosts for vaccine production.

細胞株の由来

Monkey African Green kidney

細胞株の説明

Established from the kidney of a normal adult African Green monkey. Susceptible to a wide range of viruses including polio, rubella, arboviruses and reoviruses.

アプリケーション

VERO cell lines have been used:

• in the propagation of Chandipura virus (CHPV)
• in the cytotoxicity assay to evaluate the antioxidant and anti-viral activities of Capsicum annuum methanolic extracts
• for the propagation of T.cruzi

Virus studies: polio, rubella, arboviruses and reoviruses

培地

(D6546) + 2 mM (G7513) + 10% (F2442).

継代と培養方法

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37 °C.

その他情報

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