AB-1 HA

12070506

• 別名: AB 1 HA, AB-1HA
• UNSPSCコード: 41106514

特性

• 由来生物: mouse mesothelium
• 成長モード: Adherent
• 核型: Not specified
• 形態: Epithelial; cells are small and can be spindly at low density but form a uniform cobblestone epithelial layer at near confluence
• 受容体: Not specified
• 関連疾患: cancer

詳細

細胞株の由来

Mouse mesothelioma cell line transfected with influenza haemagglutinin gene (HA)

細胞株の説明

AB-1 HA is a mouse mesothelioma cell line transfected with influenza haemagglutinin gene (HA). Mice (BALB/c strain, female, 6-8 weeks old) were exposed to crocidolite asbestos through intraperitoneal injection, resulting in tumour development. Cultures were established from malignant mesothelial cells obtained from ascites fluid. The resulting celll ine, designated AB-1 was transfected with influenza haemagglutinin gene (HA) in order to generate an endogenous tumour antigen. Cell Bank Australia reference number: CBA1374.

DNAプロファイル

特記事項なし

培地

RPMI 1640 (R0883) (with 2 mM L-Glutamine (G7513) + 25mM HEPES) + FBS / FCS (F2442) + 400ug/ml G418.

継代と培養方法

Optimal split ratio 1:10 - 1:20 (seeding density 0.7 x 10⁴ cells/cm²). Cells incubated at 37 °C with 5% CO₂. Harvest the cells using 0.05% Trypsin/EDTA at 37 °C for 5 min. Cells will require passaging twice weekly at this seeding density.

その他情報

HPA Culture Collections由来でSIGMAによって供給される培養物は研究の目的のみに使用できます。商業目的での細胞株の使用に関する質問は、その細胞株のデポジターに照会されます。一部の細胞株には、細胞株の供給の前に受給予定者によって物質移動契約書への記入を完了させるという要求など、さらなる特別な公開条件があります。詳しくはTerms & Conditions of Supplyをご覧ください。

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Depositor and originator: Richard Lake - University of Western Australia, Australia