HT29-MTX-E12

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 12040401, human colon, Epithelial

• 別名: HT-29-MTX-E12 Cells, HT29, HT29 Cells, HT29-MTX, HT29-MTX Cells, MTX-E12 Cells
• UNSPSCコード: 41106514

特性

• 由来生物: human colon
• 包装: tube of 5 μg 12040401-DNA-5UG; pkg of vial of cells 12040401-1VL
• 成長モード: Adherent
• 核型: Not specified
• 形態: Epithelial
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

HT29, HT29-MTX, methotrexate, mucous layer, intestinal permeation, oral drug absorption

細胞株の説明

HT29 cells were differentiated into mature goblet cells using methotrexate. Mucous-secreting HT29-MTX subclones were isolated from this cell clone and characterized with regard to tight junction formation, development of confluent monolayers and productio

DNAプロファイル

STR-PCR Data:
Amelogenin: X
CSF1PO: 11,12
D13S317: 11
D16S539: 11,12
D5S818: 11,12
D7S820: 10
THO1: 6
TPOX: 8,9
vWA: 17

培地

DMEM + 2mM Glutamine + 1% Non essential amino acids + 10% Foetal Bovine Serum (FBS).

継代と培養方法

Split subconfluent cultures (70-80%) 1:3 to 1:6 using 0.25% trypsin/EDTA; 7.5-10% CO_2; 37 °C. Suggested seeding density 2-4x10,000 cells/cm^².

その他情報

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