Mero-14

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 9100101, human lung, Spindle shaped cells

• 別名: Mero14 Cells
• UNSPSCコード: 41106514

特性

• 由来生物: human lung
• 包装: tube of 5 μg 09100101-DNA-5UG; pkg of vial of cells 09100101-1VL
• 成長モード: Adherent
• 核型: Number of chromosomes 72-75
• 形態: Spindle shaped cells
• 製品: Cytokeratins 7, 8, 18 and 19. Epithelial membrane antigen (EMA)
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

A human mesothelioma cell line derived from a malignant mesothelioma. Asbestos exposure

細胞株の説明

Mero-14 was derived from the pleural effusion from a male patient aged >60 with a history of asbestos exposure. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.

アプリケーション

Investigation of the properties of malignant mesothelioma cells and the development of new diagnostic methods.

DNAプロファイル

STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 12
D16S539: 12,13
D5S818: 9
D7S820: 10,11
THO1: 8
TPOX: 9,11
vWA: 15,18

培地

Hams F10 + 15% FCS + 2mM Glutamine

継代と培養方法

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Population doubling approx 24hrs

その他情報

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