C6-4-2

8062524, rat brain, Fibroblast

• UNSPSCコード: 41106514

特性

• product name: C6-4-2, 08062524
• 由来生物: rat brain
• 成長モード: Adherent
• 核型: modal No varied with passage
• 形態: Fibroblast
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Rat glioma x rat glioma hybrid

細胞株の説明

Pseudohexadecaploid cell line generated by fusion of the 8-bromodeoxyuridine resistant mutant rat octaploid glioma cell line C6-3-BU-8 and the 6-thioguanine-resistant mutant rat octaploid glioma cell line C6-3-TG-3. C6-4-2 cells provide a suitable model system for studying the regulation of the membrane potential by peptide hormones.

アプリケーション

electrophysiology

培地

DMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) + HAT (0.1 mM hypoxanthine, 10 μM aminopterin and 16 μM thymidine) Cells can be cultured without HAT provided they were cultured in between in HT medium for 3-4 days

継代と培養方法

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C

その他情報

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