N1E-115-1

08062511

• UNSPSCコード: 41106514

特性

• 由来生物: mouse nerve
• 成長モード: Adherent
• 核型: modal no. of chromosomes is 192 see reference
• 形態: neuronal
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Mouse neuroblastoma

細胞株の説明

A subclone of the cell line N1E-115 established by Amano et al. The cell line N1E-115 is an adrenergic cell line derived from mouse neuroblastoma C1300. (Mouse strain A/Jax). N1E-115 cells are reported to be slightly contaminated with with a cell type of the morphology of C6 glioma cells. It is recommended to use N1E-115-1, a subclone of N1E-115, that is devoid of the contamination.

アプリケーション

can be used as a neuronal model

培地

DMEM + 2 mM Glutamine + 10% Foetal Bovine Serum (FBS)

継代と培養方法

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C. Population doubling approximately 20 hours.

その他情報

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