KARPAS 384

6120602, human blood, Large clumps growing slowly in suspension

• 別名: KARPAS, 384, KARPAS-384, KARPAS384
• UNSPSCコード: 41106514

特性

• 製品名: KARPAS 384, from human blood, 06120602
• 由来生物: human blood
• 成長モード: Suspension
• 核型: Complex, refer to Dyer et al., 1993
• 形態: Large clumps growing slowly in suspension
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice

詳細

細胞株の由来

Human T-cell non-Hodgkin lymphoma

細胞株の説明

Established from peripheral blood of a 48 year-old male with widespread subcutaneous nodules. Biopsy showed high grade Non-Hodgkin lymphoma with numerous mitoses.

DNAプロファイル

特記事項なし

培地

RPMI 1640 + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS)

継代と培養方法

Doubling time approximately 60 hours. Cells grow slowly in suspension in large clumps. Maintain cultures between 3-9 x 10⁵ cells/ml; 5% CO₂; 37 °C. Thaw ampoule and perform a cell count, spin out the DMSO and resuspend in an appropriate volume of media to give a cell concentration of 4-5 x 10⁵ cells/ml. Viability may be poor on resuscitation and may decrease further within the first 48 hours, the culture may appear non-viable at this time. Clusters of cells will eventually appear but this could take 1-2 weeks, during this time large media changes are not recommended. It may take 2-3 weeks for vigorous growth to be established. At subculture perform a cell count and maintain cultures between 3-9 x 10⁵ cells/ml, 5% CO₂, 37 °C. For cryopreservation of KARPAS 384, freeze at approximately 2 x 10⁷ cells/ml in 90% FBS + 10% DMSO.

その他情報

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免責事項

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.