Huh-7D 12 Cell Line human

1042712, human liver, Epithelial

• 別名: Huh7 Cells, JTC-39 Cells, Japanese Tissue Culture-39 Cells
• UNSPSCコード: 41106514

特性

• 製品名: Huh-7D 12 Cell Line human, 01042712
• 由来生物: human liver
• 成長モード: Adherent
• 核型: That of original Huh-7
• 形態: Epithelial
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 保管温度: −196°C

詳細

詳細

Huh-712D is one of a series of stable HDAg (Hepatitis Delta Antigen)-expressing human hepatoma cell lines that have been isolated and characterised. Huh-7D is a derivative of Huh-7 cells, which favor hepatitis C virus replication. Huh-7 cells are superior hepatic cell models compared to HepG2 cells for apolipoprotein based metabolic studies.

細胞株の由来

Human hepatocellular carcinoma

細胞株の説明

Huh-712D is one of a series of stable HDAg (Hepatitis Delta Antigen)-expressing human hepatoma cell lines that have been isolated and characterised.

アプリケーション

Study of intracellular traffic of delta virus antigens and RNA, viral RNA replication and early virus assembly events, and interaction between HDV and HBV.

DNAプロファイル

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 10
D16S539: 10
D5S818: 12
D7S820: 11
THO1: 7
TPOX: 8,11
vWA: 16,18

培地

DMEM (D6546) + 2mM L-Glutamine (G7513) + 10% FBS / FCS (F2442)

継代と培養方法

Split sub-confluent cultures (70-80%) 1:3 to 1:5 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37 C. Media change twice a week

その他情報

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