C8218

Monoclonal Anti-Cdk4 antibody produced in mouse

clone DCS-31, ascites fluid

• MDL番号: MFCD00282839
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: ascites fluid
• 抗体製品タイプ: primary antibodies
• クローン: DCS-31, monoclonal
• 分子量: antigen 33 kDa
• 含みます: 15 mM sodium azide
• 化学種の反応性: rat, mouse, human
• テクニック: immunocytochemistry: suitable; immunoprecipitation (IP): suitable; microarray: suitable; western blot: 1:1,000 using a cultured human tumor cell line extract
• アイソタイプ: IgG2a
• UniProtアクセッション番号: P11802
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... CDK4(1019) ; mouse ... Cdk4(12567) ; rat ... Cdk4(94201)

詳細

詳細

Cdk4 exists, in part, as a multi-protein complex with a D-type cyclin, proliferating cell nuclear antigen (PCNA) and a protein inhibitor, p21^Cip1. Cdk4 associates separately with p16, particularly in cells lacking a functional retinoblastoma protein.

Monoclonal Anti-Cdk4 (mouse IgG2a isotype) is derived from the DCS-31 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Cyclin-dependent kinase 4 is localized in human chromosome 12q14.1.

特異性

抗体はCdk4と特異的に反応し、他のCdk分子は認識しません。

免疫原

リコンビナントヒトCdk4タンパク質。

アプリケーション

Monoclonal Anti-Cdk4 antibody was used in:

• immunoprecipitation of human melanoma cell line.
• immunofluorescence of fibrosarcoma cells.
• western blot analysis
• immunofluorescence
• immunohistochemistry

生物化学的／生理学的作用

In association with cyclins, cyclin-dependent kinases (CDKs) forms active kinase complexes which is responsible for regulating cell cycle progression in eukaryotic cells. Within the complexes, the CDKs serves a catalytic protein kinase activity. This catalytic activity is regulated by two mechanisms: protein phosphorylation and association of regulatory subunits.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable