C7488

Anti-CENP-E antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• 別名: Anti-Centromere-associated protein-E
• MDL番号: MFCD05665206
• UNSPSCコード: 12352203
• NACRES: NA.46

特性

• 由来生物: rabbit
• 結合体: unconjugated
• 抗体製品の状態: IgG fraction of antiserum
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 形状: buffered aqueous solution
• 分子量: antigen ~310 kDa
• 化学種の反応性: human
• テクニック: immunocytochemistry: suitable using human epitheloid carcinoma HeLa cell line.; microarray: suitable; western blot: 1:2,000 using whole extract of HeLa nuclear cell line
• UniProtアクセッション番号: Q02224
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... CENPE(1062)

詳細

詳細

Centromere-associated protein-E (CENP-E, 312 kDa) is a member of the kinesin superfamily of microtubule motor proteins and is an integral part of kinetochore corona fibers that link centromere to the spindle microtubules. CENP-E localizes to the kinetochore throughout all phases of mitotic chromosome movement from early prometaphase through anaphase A.

免疫原

ヒトCENP-EのC末端付近に位置するアミノ酸2545-2563に相当する合成ペプチドのKLH結合体。

アプリケーション

Anti-CENP-E antibody produced in rabbit has been used in:

• immunoblotting
• immunofluorescence
• immunocytochemistry.

生物化学的／生理学的作用

Centromere-associated protein-E (CENP-E) plays an important role in attachment of kinetochores to spindle microtubules in the alignment of chromosomes and is an essential component of mitotic checkpoint signaling cascade. It functions as a motor in the initial chromosome movement at the mitotic midzone. CENP-E is associated with minus end-directed microtubule motor activity suggesting that CENP-E might be responsible for poleward kinetochore movements in prometaphase and anaphase A. Suppression of CENP-E synthesis by antisense CENP-E yields chromosomes that are chronically mono-oriented with flattened bipolar spindles and generates spindle poles fragments. Depletion of CENP-E leads to profound checkpoint activation and long-term mitotic arrest. CENP-E has been implicated as a binding partner for the mitotic checkpoint kinase BubR1 during mitosis.

物理的形状

0.01 M PBS溶液(pH 7.4, 15 mMアジ化ナトリウム含有)。

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: nwg
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable