C6243

モノクローナル抗カテプシンB抗体 マウス宿主抗体

clone CB59-4B11, purified from hybridoma cell culture

• 別名: Anti-APPS, Anti-CPSB, Anti-RECEUP
• UNSPSCコード: 12352203
• NACRES: NA.45

特性

• 由来生物: mouse
• 結合体: unconjugated
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: CB59-4B11, monoclonal
• フォーム: buffered aqueous solution
• 分子量: antigen ~25 kDa
• 交差性: rat, human
• 濃度: ~1.5 mg/mL
• テクニック: immunohistochemistry: suitable; indirect ELISA: suitable; western blot: 4-8 μg/mL using HCT-116 cells.
• アイソタイプ: IgG1
• UniProtアクセッション番号: P07858
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... CTSB(1508) ; rat ... CTSB(64529)

詳細

詳細

Monoclonal Anti-Cathepsin B specifically recognizes human1 and rat1 cathepsin B (~ 25 kDa). The antibody epitope resides within the peptide sequence EPGYSP which corresponds to amino acids 212-217 of human cathepsin B. The antibody does not cross react with the closely related lysosomal cysteine proteases: cathepsins L, H, K, S, V, and W

The CTSB (Cathepsin B) gene encoding cathepsin B is mapped to human chromosome 8p23.1. Cathepsin B are normally localized in lysosomes of almost all mammalian cells, but under certain conditions they can be secreted from the cell that take part in local proteolysis. Cathepsin B is both an endo- and exopeptidase, composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor.

免疫原

Recombinant human cathepsin B.

アプリケーション

indirect ELISA, immunohistochemistry, In HCT-116 cells. 4-8μg/mL of antibody concentration may be used. A dilution of 1:800 in human pancreatic carcinoma cell line PANC-1 was used for immunoblotting

生物化学的／生理学的作用

Cathepsins are lysosomal proteases activation of enzyme precursors, and tumor invasion and metastasis. It is involved in the proteolysis of many proteins involved in different physiological and pathological pathways. In the brain, this protease, also known as amyloid precursor protein secretase, is responsible for the proteolytic processing of amyloid precursor protein (APP). An incomplete proteolytic processing of APP has been found as a causative factor in Alzheimer disease. Furthermore, ablated genes coding cathepsin B and L leads to enormous neuronal cell death,probably due to autophagy abnormalities. Cathepsin B has been implicated in tumor progression by virtue of localization at the invading front of the tumor. For example, blocking cathepsin B expression in human glioblastoma SNB19 cells by a stable transfection of an expression vector expressing antisense cathepsin B, caused suppression of glioblastoma-induced neovascularization.
Monoclonal Anti-Cathepsin B specifically recognizes human1 and rat1 cathepsin B (~ 25 kDa). The antibody epitope resides within the peptide sequence EPGYSP which corresponds to amino acids 212-217 of human cathepsin B. The antibody does not cross react with the closely related lysosomal cysteine proteases: cathepsins L, H, K, S, V, and W.
Cathepsins significantly takes part in the immune response, by processing antigens into peptides for its presentation through MHC class II molecules.

物理的形状

15 mMアジ化ナトリウム含有のpH 7.4の0.01 Mリン酸緩衝食塩水溶液.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable