C-39325

Fibroblast Growth Medium 2

SupplementMix containing all media supplements pre-mixed in one vial, 1 Mix for 500 ml

• 別名: Recommended with C-23220
• UNSPSCコード: 12352207
• NACRES: NA.81

特性

• 形状: aqueous solution
• 包装: pkg of 1 vial
• テクニック: cell culture | mammalian: suitable
• 輸送温度: dry ice
• 保管温度: −20°C

詳細

詳細

Fibroblast Growth Medium 2 SupplementMix (basal medium sold separately)

To form a complete medium, it should be combined with the basal medium: C-23220

アプリケーション

Fibroblast Growth Medium 2 is a low-serum medium and has been developed for the in vitro cultivation of adult Human Fibroblasts. The medium is optimized for primary human cells, but can also be used for bovine, porcine, and rat fibroblasts, as well as fibroblast cell lines.Recommended for:Normal Human Dermal Fibroblasts (NHDF), adult donorHuman Pulmonary Fibroblasts (HPF)Human Aortic Adventitial Fibroblasts (HAoAF)Human Uterine Fibroblasts (HUF)

構成

Part of a Cell Culture System offered in a variety of formats:Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMixGrowth Medium Kit: Includes Basal Medium and SupplementPackSupplementMix: Contains all media supplements premixed in one vialSupplementPack: Contains all media supplements as individual vials

品質

All lots of PromoCell Fibroblast Growth Medium 2 are subjected to comprehensive quality control tests using primary human adult fibroblasts. Each lot is checked for growth promoting activity, adherence rate, and typical morphology of the tested adult fibroblasts. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

調製ノート

Final supplement concentrations after addition to the basal medium:Fetal Calf Serum: 0.02 ml / mlBasic Fibroblast Growth Factor (recombinant human): 1 ng / ml Insulin: 5 µg / ml

保管および安定性

Store the SupplementMix at -20°C in the dark immediately after arrival. If stored properly, it is stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.

継代と培養方法

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安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3