C1862

モノクロナール抗コイリン マウス宿主抗体

~1.5 mg/mL, clone pδ, purified from hybridoma cell culture

• MDL番号: MFCD03792415
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: purified from hybridoma cell culture
• 抗体製品タイプ: primary antibodies
• クローン: pδ, monoclonal
• 形状: buffered aqueous solution
• 分子量: antigen ~80 kDa by SDS-PAGE
• 化学種の反応性: human
• 濃度: ~1.5 mg/mL
• テクニック: immunocytochemistry: suitable; microarray: suitable; western blot: 1-2 μg/mL using HeLa nuclear extract
• アイソタイプ: IgG1
• UniProtアクセッション番号: P38432
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... COIL(8161)

詳細

詳細

Monoclonal Anti-Coilin (mouse IgG1 isotype) is derived from the pδ hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with coilin. Coilin contains two nuclear localization sequences (NLS) (at amino acid 107-112 and 181-198) and several serine residues that are phosphorylated in vivo. The description of specific intranuclear structures known today as Cajal bodies was first published in 1903 by the neuro-cytologist Ramon-γ-Cajal who discovered that neurons contained spherical structures of around 0.5 μm in diameter that were often associated with nucleoli, nucleolar accessory bodies. It was found that patients with auto-antibodies against coiled bodies recognize a protein of 80 kDa termed p80-coilin. Nuclear antigens shown to colocalize with p80 coilin in Cajal bodies include basal transcription factors, cell cycle factors (cdks), splicing snRNPs and nucleolar factors including snoRNP.

特異性

The antibody recognizes the C-terminal region of human coilin and does not recognize mouse coilin.

免疫原

ヒトコイリンC末端(389アミノ酸)。

アプリケーション

Monoclonal Anti-Coilin antibody produced in mouse has been used in:

• immunoblotting
• immunocytochemistry
• cell microinjection
• fluorescence imaging
• indirect immunofluorescence

生物化学的／生理学的作用

Mutating Serine-202 to Aspartate causes the disappearance of coiled bodies and a redistribution of coilin to intranucleolar domains. Coilin plays a key role in ribonucleoprotein and Cajal body formation.

物理的形状

0.01M PBS溶液 (pH 7.4, 15mMアジ化ナトリウム含有)。

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: nwg
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable