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テクニック
cell culture | mammalian: suitable
輸送温度
dry ice
保管温度
−20°C
詳細
Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details. Cells are dissolved in RNAlater for subsequent RNA, DNA, or protein analysis. Note that cell pellets cannot be revived.
PromoCell Cell Pellets are stored in RNAlater® - an aqueous protection solution that rapidly permeates the cells and stabilizes cellular DNA, RNA, and proteins and protects them from degradation. This makes the PromoCell Cell Pellets very useful for a wide range of research applications like gene expression analysis, cell characterization studies, and protein characterization experiments.
Note: RNAlater® denatures proteins. Therefore, proteins will be suitable for e.g. Western blotting but not for applications that require native proteins. Because of the density of RNAlater® Solution, greater centrifugal forces are required to pellet cells. We recommend to transfer the content of the vial into a 15 ml conical tube, to add an equal volume of PBS and to spin down the cells at 5000 x g for 10 minutes at 4°C. Each Cell Pellet is made from 1 million cells and is dissolved in 200 µl RNAlater®. The Cell Pellets are produced from released cell lots and have the same characteristics, i.e. expression profile or cell type specific antigens. On request, viable cells from the same lot are available for cultivation.
PromoCell Cell Pellets are stored in RNAlater® - an aqueous protection solution that rapidly permeates the cells and stabilizes cellular DNA, RNA, and proteins and protects them from degradation. This makes the PromoCell Cell Pellets very useful for a wide range of research applications like gene expression analysis, cell characterization studies, and protein characterization experiments.
Note: RNAlater® denatures proteins. Therefore, proteins will be suitable for e.g. Western blotting but not for applications that require native proteins. Because of the density of RNAlater® Solution, greater centrifugal forces are required to pellet cells. We recommend to transfer the content of the vial into a 15 ml conical tube, to add an equal volume of PBS and to spin down the cells at 5000 x g for 10 minutes at 4°C. Each Cell Pellet is made from 1 million cells and is dissolved in 200 µl RNAlater®. The Cell Pellets are produced from released cell lots and have the same characteristics, i.e. expression profile or cell type specific antigens. On request, viable cells from the same lot are available for cultivation.
アプリケーション
Primary Normal Human Epidermal Melanocytes (NHEM) are isolated from the epidermis of juvenile foreskin and are provided in a proliferating format. Melanocytes are located in the stratum basale, but branch out between the keratinocytes in suprabasal layers. About 5-10 % of the cells in the epidermis are melanocytes. The main purpose of melanocytes is the production of melanin, the protein responsible for the pigmentation of the skin, eyes, and hair. Melanin protects the cells in the skin and in deeper layers from the hazardous effects of UV radiation. It is produced and stored in melanosomes, which are located close to the melanocyte membrane, but it can also be transferred to neighboring keratinocytes.
Normal Human Epidermal Melanocytes M3 are isolated using the serum-free, BPE-free and PMA-free Melanocyte Growth Medium M3. Since PMA is a tumor promoting mitogen, it can interfere with experimental approaches. Therefore, we recommend using cells isolated in Melanocyte Growth Medium M3 and also using this medium for cultivation. Normal Human Epidermal Keratinocytes (NHEK) and Normal Human Dermal Fibroblasts (NHDF) from the same donor are available on request.
Normal Human Epidermal Melanocytes M3 are isolated using the serum-free, BPE-free and PMA-free Melanocyte Growth Medium M3. Since PMA is a tumor promoting mitogen, it can interfere with experimental approaches. Therefore, we recommend using cells isolated in Melanocyte Growth Medium M3 and also using this medium for cultivation. Normal Human Epidermal Keratinocytes (NHEK) and Normal Human Dermal Fibroblasts (NHDF) from the same donor are available on request.
品質
Rigid quality control tests are performed for each lot of the corresponding cells. They are tested for cell viability, cell morphology, growth performance and for cell-type specific markers. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).
継代と培養方法
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その他情報
The Cell Pellets can be stored indefinitely at -20°C, and for up to 1 month at 4°C. Note: The Cell Pellets will not freeze at -20°C. Always centrifuge product briefly before opening vial.
法的情報
RNAlater is a registered trademark of Ambion, Inc.
保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
C-14044:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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