B9428

モノクローナル抗Bin1抗体 マウス宿主抗体

clone 99D, ascites fluid

• 別名: 抗SH3P9抗体, 抗ampl抗体, 抗アンフィフィシンII抗体
• MDL番号: MFCD02095896
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: mouse
• 結合体: unconjugated
• 抗体製品の状態: ascites fluid
• 抗体製品タイプ: primary antibodies
• クローン: 99D, monoclonal
• 分子量: antigen 65-75 kDa
• 含みます: 15 mM sodium azide
• 化学種の反応性: rabbit, mouse, rat, avian, human
• テクニック: flow cytometry: suitable; immunocytochemistry: suitable using nucleoplasm, subnuclear punctate compartment, and cytoplasm; immunohistochemistry (frozen sections): suitable; immunoprecipitation (IP): suitable; indirect ELISA: suitable; western blot: 1:50,000 using a rat brain cytosol preparation
• アイソタイプ: IgG2b
• UniProtアクセッション番号: O00499
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... BIN1(274) ; mouse ... Bin1(30948) ; rat ... Bin1(117028)

詳細

詳細

Monoclonal Anti-Bin1 (SH3P9, Amphiphysin II, amphl) (mouse IgG2b isotype) is derived from the 99D hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a recombinant polypeptide, containing amino acids 189-398 of human Bin1. Bin1 [Box-dependent myc-interacting protein-1, also known as SH3P9, amphiphysin II and amphl (amphiphysin-like)] is a novel protein that has features of a tumor suppressor. The human BIN1 gene has been mapped to chromosome 2q14. In human and rodent cells it appears as a short-lived (half-life of approx. 2 hrs) monomeric phosphoprotein (approx. 70 kDa). Bin1 molecule appears in different isoforms or conformations, and the localization varies between normal and tumor cells. In normal cells, Bin1 is predominantly nucleoplasmic, but is also present in a subnuclear compartment. In a panel of tumor cells that express Bin1, the predominant localization of the molecule is in the subnuclear compartment.

特異性

抗体は、ヒトBin1分子のmyc結合ドメインの33残基セグメント(MBD, アミノ酸 323-356)と特異的に反応します。Bin1に関連する45 kDaのポリペプチドとは反応しません。

免疫原

ヒトBin1のアミノ酸189-398に相当するリコンビナントポリペプチド。

アプリケーション

Monoclonal Anti-Bin1 antibody produced in mouse has been used in:

• western blotting
• enzyme linked immunosorbent assay (ELISA)
• immunoprecipitation
• immunocytochemistry
• immunohistochemistry
• flow cytometry

生物化学的／生理学的作用

Box-dependent myc-interacting protein-1 (Bin1) interacts with and inhibits the oncogenic activity of the myc oncoprotein that has a major role in many human cancers. The loss of Bin1 may contribute to growth deregulation in cancer cells in carcinoma of the breast, colon, lung, cervix, prostate and liver. In bin1 locus mid-2q region that is deleted in approx. 42% of metastatic prostate cancers, and at the syntenic murine locus, in >90% of radiation-induced myeloid leukemias. In addition to the role of Bin1 in controlling neoplastic cell growth and cell cycle by the inhibition of transformation by both myc and the adenovirus E1A protein. Deficits in expression are functionally significant, because ectopic Bin1 can inhibit the growth of tumor cells that lack endogenous expression. Bin1 has also been found to be highly expressed in murine skeletal muscle; overexpression of Bin1 promotes myotube formation and upregulation of myosin heavy chain, while interference with Bin1 expression significantly impairs these processes.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable