B8429

モノクロナール抗Bax マウス宿主抗体

clone 6A7, purified from hybridoma cell culture

• 別名: Anti-BCL2L4
• MDL番号: MFCD00282585
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: mouse
• 結合体: unconjugated
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: 6A7, monoclonal
• 形状: buffered aqueous solution
• 分子量: antigen 21 kDa (and possibly a dimer of 42 kDa)
• 化学種の反応性: mouse, rat, human
• 濃度: 2 mg/mL
• テクニック: immunoprecipitation (IP): suitable; microarray: suitable; western blot: 10-60 μg/mL using extract of human breast adenocarcinoma MCF-7 cells, activated with dexamethasone
• アイソタイプ: IgG1
• UniProtアクセッション番号: Q07812
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... BAX(581) ; mouse ... Bax(12028) ; rat ... Bax(24887)

詳細

詳細

Bax is a 21kD integral organelle membrane protein that belongs to the Bcl-2 family and is expressed mainly in the mitochondria. The formation of bax homodimer accelerates apoptotic death whereas its heterodimerization with Bcl-2 or Bcl-xL can block apoptosis. Monoclonal anti-Bax antibody can be used to study the intracellular redistribution of Bax protein upon induction of apoptosis and its unique subcellular localization. This product can also be used in immunoblot analysis to estimate variations in the expression of specific proteins involved in apoptosis signalling. Mouse anti-Bax antibody reacts specifically with the epitope present within the amino acids 12-24 shared by bax from rat, mouse and human.

The B-cell lymphoma 2 (BCL2) associated X, apoptosis regulator (BAX) is mapped to human chromosome 19q13.33.

免疫原

ヒトBax配列のアミノ酸12-24に相当する合成ペプチドのKLH結合体。

アプリケーション

Anti-Bax antibody, Mouse monoclonal has been used in the western blotting detection in human retinal pigment epithelium cell lines, glioma, and breast cancer cell lines.

Monoclonal anti-Bax antibody can be used in immunocytochemistry (diluted 1: 1500) and western blot. It is also useful in microarray and immunoprecipitation.

生物化学的／生理学的作用

Mutations in the B-cell lymphoma 2 (BCL2) associated X, apoptosis regulator (BAX) gene is implicated in colorectal cancer. BAX is associated with ovarian follicular dysfunction.

物理的形状

PBS溶液 (pH 7.4, 15mMアジ化ナトリウム含有)。

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: nwg
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable