A7481

抗Atg1/ULK1 ウサギ宿主抗体

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

• 別名: UNC51, Unc-51 like kinase 1 (C. elegans), Unc51.1
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: rabbit
• 結合体: unconjugated
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 形状: buffered aqueous solution
• 分子量: antigen ~150 kDa
• 化学種の反応性: rat, human (predicted), mouse
• 強化検証: recombinant expression; Learn more about Antibody Enhanced Validation
• 濃度: ~1 mg/mL
• テクニック: western blot: 0.5-1.0 μg/mL using whole extracts of HEK-293T cells expressing mouse ULK1; western blot: 3-6 μg/mL using whole extracts of rat PC12
• UniProtアクセッション番号: O75385
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... ULK1(8408) ; mouse ... Ulk1(22241) ; rat ... Ulk1(360827)

詳細

詳細

Atg1 is an autophagy protein that functions as a serine/threonine kinase in yeast to regulate autophagasome formation. Atg1 is ULK1 in mammals.

Unc-51 like autophagy activating kinase 1 (ULK1) gene is located on human chromosome 12q24.33.

特異性

Anti- Atg1/ULK1 recognizes rat and mouse Atg1/ULK1.

免疫原

Synthetic peptide corresponding to amino acids of mouse Atg1/ULK1 conjugated to KLH via a cysteine residue. The corresponding sequence is identical in rat and differs by 3 amino acids in human.

アプリケーション

Anti-Atg1/ULK1 antibody produced in rabbit has been used in western blotting and immunohistochemical staining.

生物化学的／生理学的作用

Unc-51 like autophagy activating kinase 1 (ULK1) regulates autophagy initiation and innate immunity of interferons (IFNs). ULK1 is critical for neuronal development. It is essential for neurite elongation and differentiation of cerebellar granule neurons. Mutations in this gene is linked to latent mycobacterium tuberculosis infection (LTBI).

物理的形状

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

保管および安定性

For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)