A2429

抗マウスIgG (Fc特異的)-アルカリフォスファターゼ ヤギ宿主抗体

affinity isolated antibody, buffered aqueous solution

• 別名: Goat Anti-Mouse IgG (Fc specific)–AP
• MDL番号: MFCD00162626
• UNSPSCコード: 12352203
• NACRES: NA.46

特性

• 由来生物: goat
• 結合体: alkaline phosphatase conjugate
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: secondary antibodies
• クローン: polyclonal
• 形状: buffered aqueous solution
• 化学種の反応性: mouse
• 以下との反応禁止: bovine, human, horse
• テクニック: direct ELISA: 1:30,000; immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40; western blot (chemiluminescent): 1:160,000-1:320,000
• 輸送温度: wet ice
• 保管温度: 2-8°C
• ターゲットの翻訳後修飾: unmodified

詳細

詳細

Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein. IgG is a major class of immunoglobulin. Mouse consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3.

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. IgG1 regulates complement fixation in mice.

Mouse IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in mouse serum. IgG crosses the placental barrier, is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection.

免疫原

Purified mouse IgG, Fc fragment

アプリケーション

PBS/0.1%Tweenおよび1%BSAで1：1000に希釈し、37°Cで1.5時間、ELISAアッセイでアルカリホスファターゼ結合ヤギ抗マウスFc特異抗体を二次抗体として使用しました。37°Cで30分間、基質として4-ニトロフェニルホスフェート（Sigma）を使用して抗体を開発しました。

Anti-Mouse IgG (Fc specific)-Alkaline Phosphatase antibody is suitable for use in ELISA (1:1500) and western blot. The antibody can also be used for immunohistochemistry (1:40 using formalin-fixed, paraffin-embedded sections).

Anti-Mouse IgG (Fc specific)-Alkaline Phosphatase antibody produced in goat has been used in enzyme-linked immunosorbent assay (ELISA), sandwich ELISA and enzyme-linked immune absorbent spot (ELISPOT).

The product has been used as a secondary antibody for detecting mouse serum anti-hemagglutinin (HA) antibodies using ELISA techniques.

生物化学的／生理学的作用

Immunoglobulin G (IgG) participates in hypersensitivity type II and type III reactions. IgG helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.

その他情報

本抗体は、ウシ, ウマ, ヒト血清タンパク質で吸収済です。

物理的形状

0.05M Tris溶液 (pH 8.0, 1%BSA, 1mM MgCl₂ , 15mMアジ化ナトリウム含有)

調製ノート

ウシ, ウマ, ヒトサンプルでのバックグラウンド染色の低減のため吸収処理済み。

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable