A5463

抗アセチル化タンパク質抗体 ウサギ宿主抗体

~0.5 mg/mL, affinity isolated antibody, buffered aqueous solution

• MDL番号: MFCD03454689
• UNSPSCコード: 12352203

特性

• 由来生物: rabbit
• 結合体: unconjugated
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 形状: buffered aqueous solution
• 化学種の反応性: human, chicken, bovine
• 濃度: ~0.5 mg/mL
• テクニック: indirect immunofluorescence: 1:1,000 using methanol/acetone-fixed chicken fibroblats cells; microarray: suitable; western blot: 1:1,000 using Whole cell extract of human epitheloid carcinoma HeLa cell line treated with sodium butyrate
• 輸送温度: dry ice
• 保管温度: −20°C

詳細

詳細

アセチル化ヒストンH4 [12 kDa]、アセチル化H1およびH2bやアセチル化BSAなど複数のアセチル化タンパク質と反応します。アセチル化ヒストンH3は認識されません。

Protein acetylation on lysine is a reversible protein modification that regulates protein functions. Histone acetylation at N-epsilon-lysine residues can destabilize the nucleosome structure and render DNA more accessible to transcription factors. Protein acetylation controls the function of transcription factors such as p53, GATA-1, E2F, TFIIEb, TFIIF, and EKLF.
Staining of histone H4 in immunoblotting is specifically inhibited with acetylated histone H4 immunizing peptide (human, amino acids 1-20 with N-epsilon-acetylated lysines at positions 5, 8, 12, and 16). No inhibition with the non-acetylated histone H4 peptide (human, amino acids 1-20) is observed.

免疫原

ヒトヒストンH4のN末端アミノ酸配列1-20 (5, 8, 12, 16番目はN-ε-アセチル化リジン)に相当する合成アセチル化ペプチドのKLH結合体。

アプリケーション

Rabbit anti-acetylated protein antibody can be used for microarrays, indirect immunofluorescence (1:1,000) and western blot (1:1,000) assays.

物理的形状

0.01M PBS溶液 (pH 7.4, 1%BSA, 15mMアジ化ナトリウム含有)。

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.