A4108

アミノフェニルフルオレセイン溶液

5 mM in DMF, suitable for fluorescence

• 別名: 2-[6-(4'-アミノ)フェノキシ-3H-キサンテン-3-on-9-イル]安息香酸溶液, APF溶液
• EC Number: 200-679-5
• UNSPSCコード: 12352108
• NACRES: NA.32

特性

• フォーム: liquid
• 品質水準: 200
• 濃度: 5 mM in DMF
• 適合性: suitable for fluorescence
• 保管温度: 2-8°C

詳細

詳細

Aminophenyl fluorescein solution, also known as APF, is a novel fluorescent probe. APF exists as a non-fluorescent entity, which converts to its fluorescent form called fluorescein upon oxidation. The excitation length is at 488nm, and the emission spectrum is monitored between 495-600nm and measured using flow cytometry.

Fluorescence intensity increases after reaction with hROS (e.g. hydroxy radical, peroxynitrite and hypochlorite). And, APF is resistant to light-induced autoxidation.

アプリケーション

Aminophenyl fluorescein solution is a suitable tool to detect hypobromous acid (HOBr) produced in eosinophils. It is used to detect the halogenating activity of heme peroxidases in leukocytes. APF does not react with nitric oxide, superoxide, and hydrogen peroxide; hence, it can be used to detect peroxynitrite differentially.

高活性酸素種(hROS)の蛍光検出試薬です。hROS(例えば、ヒドロキシラジカル、ペルオキシナイトライト、次亜塩素酸塩)と反応すると、蛍光強度が増加します。APFは一酸化窒素、スーパーオキシド、過酸化水素と反応しないため、ペルオキシナイトライトを特異的に検出できます。APFは光誘導自動酸化に対して安定です。

安全性情報

• ピクトグラム: GHS02,GHS08,GHS07
• シグナルワード: Danger
• 危険有害性情報: H226,H312 + H332,H319,H360D
• 注意書き: P210 - P280 - P303 + P361 + P353 - P304 + P340 + P312 - P305 + P351 + P338 - P308 + P313
• 危険有害性の分類: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Eye Irrit. 2 - Flam. Liq. 3 - Repr. 1B
• 保管分類コード: 3 - Flammable liquids
• WGK: WGK 2
• 引火点(°F): 135.5 °F - closed cup
• 引火点(℃): 57.5 °C - closed cup
• 個人用保護具 (PPE): Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter