A3429

Differentiation Solution

for the differentiation of regressive hematoxylin stains

• 別名: 酸アルコール
• MDL番号: MFCD00243254
• UNSPSCコード: 12171500
• NACRES: NA.47

特性

• 品質水準: 200
• フォーム: solution
• シェルフライフ: 40 mo.
• 色: colorless
• アプリケーション: hematology; histology
• 保管温度: room temp

詳細

詳細

Differentiation solution comprises acidified ethanol or isopropanol that is used to decolorize hematoxylin stain. The duration of exposure to the solution determines the degree of decolorization. Differentiation is a crucial step in hematoxylin-eosin staining to achieve the proper color contrast necessary for visualization, by selectively removing excess hematoxylin from the tissue section and leaving behind the desired staining intensity.

アプリケーション

Differentiation solution is employed mainly for the differentiation of regressive hematoxylin stains. It has been used in the following studies:

• the prediction of pulmonary metastasis progression in osteosarcoma patients
• the development of multispectral imaging to detect melanin

ヘマトキシリン退行性染色の分別に使用する酸性化アルコール溶液。

特徴および利点

• Desired staining patterns can be obtained by varying the degree of exposure to the differentiation solution.
• Results are sharper and more rapid compared to other differentiation methods.
• Crisp and clear differentiation between cellular structures is obtainable when performed according to Sigma-Aldrich Procedure No. GHS.

原理

In regressive hematoxylin staining, a highly concentrated hematoxylin solution is used to rapidly overstain both nuclei and cytoplasm, and the cytoplasm is subsequently decolorized and excess hematoxylin is removed from the nucleus with dilute acid. Improper differentiation leaves excess residual hematoxylin that obscures the fine details of nuclear structures and chromatin and prevents eosin uptake.

安全性情報

• ピクトグラム: GHS02,GHS08,GHS05
• シグナルワード: Danger
• 危険有害性情報: H225,H290,H319,H371
• 注意書き: P210 - P233 - P234 - P240 - P305 + P351 + P338 - P308 + P311
• 危険有害性の分類: Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1 - STOT SE 2
• ターゲットの組織: Eyes,Central nervous system
• 保管分類コード: 3 - Flammable liquids
• WGK: WGK 1
• 引火点(°F): 71.6 °F
• 引火点(℃): 22 °C