A2951

モノクロナール抗アルカリフォスファターゼ, ヒト胎盤 マウス宿主抗体

clone 8B6, ascites fluid

• 別名: モノクロナール抗アルカリフォスファターゼ, ヒト胎盤, 抗ホスファターゼ, アルカリ性(ヒト胎盤)抗体
• MDL番号: MFCD00162106
• UNSPSCコード: 12352203
• NACRES: NA.46

特性

• 由来生物: mouse
• 結合体: unconjugated
• 抗体製品の状態: ascites fluid
• 抗体製品タイプ: primary antibodies
• クローン: 8B6, monoclonal
• 含みます: 15 mM sodium azide
• 化学種の反応性: human
• テクニック: immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:4,000 using human placenta; immunohistochemistry (frozen sections): suitable; indirect ELISA: suitable using whole cells; radioimmunoassay: suitable using radio-immunolocalization of xenografts of human cell lines expressing PLAP in nude mice²; western blot: suitable
• アイソタイプ: IgG2a
• UniProtアクセッション番号: P05187
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... ALPP(250)

詳細

詳細

SDSゲルにおいて、この製品はヒト胎盤アルカリホスファタ-ゼのReganおよびNagaoアイソザイムと反応します(hPLAP, 130 kDa, 67/130 kDa)。RIAによるこの抗体とhPLAPの結合親和定数は、5× 10⁹LM^-1です。PLAP様酵素とは反応しません。

Monoclonal Anti-Human Placental Alkaline Phosphatase (mouse IgG2a isotype) is derived from the 8B6 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. Placental alkaline phosphatase (PLAP) or ALPP is a phosphomonoesterase. It is expressed in the placenta and endometrium. The PLAP gene is localized on human chromosome 2q37.1. Small amounts of hPLAP are found in the endocervix, fallopian tubes and lung. Very small amounts of heat-stable AP resembling hPLAP (hPLAPlike AP) are expressed in the testis, thymus and in rare colon epithelial cells.

免疫原

胎盤アルカリホスファタ-ゼを発現するヒト類表皮癌細胞

アプリケーション

Anti-Alkaline phosphatase human placental antibody coupled to agarose was packed in a column and used to purify alkaline-phosphatase conjugated FGF receptor out of conditioned media from COS-7 cells. Column was washed with 1M NaCl.

Cultured pancreatic islet cells were fixed with cold 4% paraformaldhyde at room temperature for 10 minutes and incubated with mouse monoclonal anti-Alkaline Phosphatase, Human Placental antibody at 1:500. Mouse monoclonal anti-Alkaline Phosphatase, Human Placental antibody was used to detect a unique molecular probe that consisted of the FGFR extracellular domain expressed as a chimera with human PLAP.

Monoclonal Anti-Alkaline Phosphatase, Human Placental antibody produced in mouse has been used in:

• enzyme-linked immunosorbent assay (ELISA)
• immunocytochemistry
• radio-immunolocalization
• western blot
• immunofluorescence
• immunohistochemistry

生物化学的／生理学的作用

Placental alkaline phosphatase (PLAP) or ALPP is a hydrolase, which is involved in the hydrolysis of phosphomonoesters. It has been shown to be a biomarker for ovarian cancer. The protein is expressed in carcinomas such as ovarian adenocarcinoma, dysgerminoma, serous cystadenocarcinoma and undifferentiated carcinoma. Although hPLAP has been analyzed as a potential tumor marker, it has not been widely used in the routine clinical laboratory because of its low overall specificity due to heat-inactivation, cross-reactivity with intestinal AP and L-phenylalanine sensitivity. The use of monoclonal antibodies as reagents has greatly improved the sensitivity, reproducibility and accuracy of the tests used to distinguish placental isozymes from their tumor cell counterparts. Thus, monoclonal anti-alkaline phosphatase may be useful as diagnostic tools for tumor detection.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable