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由来生物
mouse
品質水準
結合体
unconjugated
抗体製品の状態
ascites fluid
抗体製品タイプ
primary antibodies
クローン
2G11, monoclonal
形状
buffered aqueous solution
分子量
antigen 60-80 kDa (glycosylated form)
化学種の反応性
pig, mouse, rabbit, rat, bovine, ferret, canine
テクニック
immunohistochemistry (frozen sections): suitable
indirect immunofluorescence: 1:2,000
western blot (chemiluminescent): 1:4,000
アイソタイプ
IgG1
UniProtアクセッション番号
輸送温度
dry ice
保管温度
−20°C
ターゲットの翻訳後修飾
unmodified
遺伝子情報
mouse ... Atp4b(11945)
rat ... Atp4b(24217)
詳細
抗体は、さまざまな生物種のH+/K+ ATPア-ゼβサブユニットと反応します。βサブユニットの細胞質側に位置するアミノ酸1-13または15-28内に存在するエピト-プと結合します。抗体は、H+/K+-ATPア-ゼの酵素活性を阻害するとともに、細胞質のK+結合部位の親和性を変化させることが確認されています。H+/K+ ATPア-ゼβサブユニットの存在の特定および検出に利用することができます。
The H+/K+-ATPase is a gastric proton pump that is a member of the P-type cation-transporting adenosine 5′-triphosphatase (ATPase) family. H+/K+-ATPase consists of a large transmembrane, catalytic α subunit, that contains sites for ATP binding and phosphorylation. This ATPase also has a smaller glycoprotein (β) subunit which may play a role in maintaining the structural and functional integrity of the complex. Mouse monoclonal anti-H+/K+ ATPase (β subunit) binds to H+/K+-ATPase β subunit in cow, dog, pig, rabbit, mouse, ferret and rat tissues. By immunoblotting, the antibody detects various forms of the β subunit, including a 60-80kDa glycosylated protein, a 52kDa β subunit precursor and the 34kDa core peptide.
免疫原
脱グリコシル化したブタ胃ミクロソ-ム由来H+/K+ ATPア-ゼのコアを形成する34 kDa βサブユニット。
アプリケーション
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western Blotting (1 paper)
Mouse monoclonal anti-H+/K+ ATPase (β subunit) antibody can be used for indirect immunofluorescence (1:2,000), chemiluminescent western blot (1:4,000), and immunohistochemical applications.
物理的形状
PBS溶液 (0.05%アジ化ナトリウム含有)。
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
10 - Combustible liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
個人用保護具 (PPE)
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
A274-VAR:
A274-100UL:
A274-100UL-PW:
A274-BULK:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Ivana Novak et al.
The Journal of biological chemistry, 286(1), 280-289 (2010-10-28)
Pancreas secretes fluid rich in digestive enzymes and bicarbonate. The alkaline secretion is important in buffering of acid chyme entering duodenum and for activation of enzymes. This secretion is formed in pancreatic ducts, and studies to date show that plasma
Salim M A Bastaki et al.
Molecular and cellular biochemistry, 341(1-2), 43-50 (2010-03-20)
Gastric pathology is a common complication in diabetes mellitus. The aim of the study was to evaluate the functions and morphological changes of the parietal cells of the rat stomach after streptozotocin-induced diabetes. Diabetes mellitus was induced in Wistar rats
D C Chow et al.
The American journal of physiology, 265(6 Pt 1), C1562-C1570 (1993-12-01)
The gastric proton pump, H(+)-K(+)-ATPase, is composed of alpha- and beta-subunits. The 95-kDa alpha-subunit has been referred to as the catalytic subunit containing sites for ATP binding and phosphorylation. The beta-subunit is a glycoprotein with a 34-kDa core peptide that
Marco Tozzi et al.
Cancers, 12(3) (2020-03-14)
Pancreatic duct cells are equipped with acid/base transporters important for exocrine secretion. Pancreatic ductal adenocarcinoma (PDAC) cells may utilize such transporters to acidify extracellular tumor microenvironment, creating a niche favoring cell proliferation, fibrosis and resistance to chemotherapy-all contributing to the
Jing Wang et al.
PloS one, 10(5), e0126432-e0126432 (2015-05-21)
The mechanism by which pancreas secretes high HCO3- has not been fully resolved. This alkaline secretion, formed in pancreatic ducts, can be achieved by transporting HCO3- from serosa to mucosa or by moving H+ in the opposite direction. The aim
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