79636

Atto 590 NHSエステル

suitable for fluorescence, BioReagent, ≥90.0% (degree of coupling)

• 実験式（ヒル表記法）: C₄₁H₄₂ClN₃O₁₁
• CAS番号: 670269-33-7
• 分子量: 788.24
• MDL番号: MFCD03456133
• UNSPSCコード: 12352108
• NACRES: NA.32

特性

• 製品種目: BioReagent
• アッセイ: ≥90.0% (HPLC); ≥90.0% (degree of coupling)
• 形状: powder
• メーカー／製品名: ATTO-TEC GmbH
• λ: in ethanol (with 0,1% trifluoroacetic acid)
• UV吸収: λ: 597-663 nm A_max
• 適合性: suitable for fluorescence
• 検出方法: fluorometric
• 保管温度: −20°C
• InChI: 1S/C41H41N3O7.ClHO4/c1-9-42-31-18-33-29(16-26(31)22(3)20-40(42,5)6)37(30-17-27-23(4)21-41(7,8)43(10-2)32(27)19-34(30)50-33)28-15-24(11-12-25(28)38(47)48)39(49)51-44-35(45)13-14-36(44)46;2-1(3,4)5/h11-12,15-21H,9-10,13-14H2,1-8H3;(H,2,3,4,5)
• InChI Key: SHJDVERDESTYRQ-UHFFFAOYSA-N

詳細

詳細

Atto 590 is a novel fluorescent label belonging to the class of Rhodamine dyes, which has an absorption maximum of 594 nm and an emission maximum of 624 nm. Atto 590-N-hydroxysuccinimide (NHS) is membrane permeable.

Atto 590 is a novel fluorescent label belonging to the class of Rhodamine dyes. The dye is designed for application in the area of life science, e.g. labeling of DNA, RNA or proteins. The Atto dyes are a series of fluorescent dyes that provide all the crucial properties required for modern fluorescent technologies, such as fluorescence microscopy, flow-cytometry, fluorescence in situ hybridization (FISH), receptor binding assays or enzyme assays. The dye is highly suitable for single-molecule detection applications and high-resolution microscopy. The NHS-esters are used in common conjugation protocols.

アプリケーション

Atto 590 NHS ester may be suitable for use in site-specific labeling of human embryonic kidney (HEK293T) cell lysates for western blotting, fluorescence, and widefield microscopy studies.

Molar absorption 120,000 1/M cm, abs: 593 nm, em: 620 nm, QY=0.93, T_fl 4.0 ns (unpublished data)

特徴および利点

• Absorption maxima cover a range from 590 to 620 nm.
• Structural properties support extraordinary photostability.
• High fluorescence quantum yield and high thermal stability.
• The fluorescence characteristics of the dye are quite insensitive to environmental changes (e.g., pH changes). This holds for both excitation and emission wavelengths and for the decay time of their fluorescence emission.
• Compatible with common labeling procedures for proteins and amino-functionalized oligonucleotides.

その他情報

FRETによる単独の細線導波路での多段階エネルギ-移動の研究や、蛍光エネルギ-移動、FRET、走査顕微鏡に用いられます。

法的情報

本製品は研究専用です。商業目的で使用する場合は、ライセンス取得について知的所有権（IP）保持者（ATTO-TEC GmbH、ドイツ）にお問い合わせください。

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, type N95 (US)