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詳細
Abberior STAR RED is an already well known dye named also KK114 in literature (a scientific web search shows more than 60 publications, a selection is listed below). Please note the minimal background signal due to negligible unspecific binding. A key feature of the dye is its comparably long lifetime of ~3.5ns. The dye works exceptionally well with the Abberior Instruments STED microscope as well as with the Leica STED microscope.
Abberior STAR RED can substitute Atto™ 647N, AlexaFluor 647, or Cy®5. It can be excited with diode lasers (635 nm, 650 nm) or with the 647 nm line of a Krypton laser. For STED, a depletion wavelength around 750 nm is recommended. Please see reference [2] for detailed characteristics.
Best results are obtained with freshly prepared samples.
Abberior STAR RED can substitute Atto™ 647N, AlexaFluor 647, or Cy®5. It can be excited with diode lasers (635 nm, 650 nm) or with the 647 nm line of a Krypton laser. For STED, a depletion wavelength around 750 nm is recommended. Please see reference [2] for detailed characteristics.
Best results are obtained with freshly prepared samples.
アプリケーション
Abberior™ STAR RED labelled cholesterol analogs were used for STED-FCS (stimulated emission depletion - fluorescence correlation spectroscopy) based measurements of membrane diffusion dynamics. Anti-mouse secondary antibody coupled to Abberior™ STAR RED has been used for STED microscopy imaging in Vero cell line.
適合性
Designed and tested for fluorescent super-resolution microscopy
法的情報
6538 is a trademark of American Type Culture Collection
Atto is a trademark of Atto-Tec GmbH
Cy is a registered trademark of Cytiva
abberior is a registered trademark of Abberior GmbH
保管分類コード
11 - Combustible Solids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
43354-1MG:
43354-BULK:
43354-1MG-BULK:
Franziska Curdt et al.
Optics express, 23(24), 30891-30903 (2015-12-25)
Despite the need for isotropic optical resolution in a growing number of applications, the majority of super-resolution fluorescence microscopy setups still do not attain an axial resolution comparable to that in the lateral dimensions. Three-dimensional (3D) nanoscopy implementations that employ
Erdinc Sezgin et al.
Journal of lipid research, 57(2), 299-309 (2015-12-25)
Cholesterol (Chol) is a crucial component of cellular membranes, but knowledge of its intracellular dynamics is scarce. Thus, it is of utmost interest to develop tools for visualization of Chol organization and dynamics in cells and tissues. For this purpose
Daniel Neumann et al.
PMC biophysics, 3(1), 4-4 (2010-03-09)
The voltage-dependent anion channel (VDAC, also known as mitochondrial porin) is the major transport channel mediating the transport of metabolites, including ATP, across the mitochondrial outer membrane. Biochemical data demonstrate the binding of the cytosolic protein hexokinase-I to VDAC, facilitating
Christian Kukat et al.
Proceedings of the National Academy of Sciences of the United States of America, 108(33), 13534-13539 (2011-08-03)
Mammalian mtDNA is packaged in DNA-protein complexes denoted mitochondrial nucleoids. The organization of the nucleoid is a very fundamental question in mitochondrial biology and will determine tissue segregation and transmission of mtDNA. We have used a combination of stimulated emission
Roman Schmidt et al.
Nano letters, 9(6), 2508-2510 (2009-05-23)
Because of the diffraction resolution barrier, optical microscopes have so far failed in visualizing the mitochondrial cristae, that is, the folds of the inner membrane of this 200 to 400 nm diameter sized tubular organelle. Realizing a approximately 30 nm
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