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Key Documents

安全性情報

402OA-05A

Sigma-Aldrich

Human Chondrocytes - Osteoarthritis: HC-OA, adult

別名:

HC-OA cells

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About This Item

UNSPSCコード:
41106514
NACRES:
NA.81

由来生物

human articular cartilage (osteoarthritic)

品質水準

包装

pkg of 500,000 cells

メーカー/製品名

Cell Applications, Inc

成長モード

Adherent

核型

2n = 46

形態

chondrocyte

テクニック

cell culture | mammalian: suitable

関連疾患

arthritis

輸送温度

dry ice

保管温度

−196°C

詳細

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.

HC-OA Human Chondrocytes are derived from human articular cartilage of donors with osteoarthritis (OA). Osteoarthritis is an inflammatory disease characterized by increased degradation of cartilage tissue in the joint due to overproduction of enzymes degrading the extracellular matrix. Despite the initial proliferation and activation of chondrocytes, they are not able to efficiently repair the degrading cartilage. Instead, chondrocytes undergo terminal differentiation and eventually apoptose, leading to mineralization of cartilage in a process resembling bone formation during development. Thus, HC-OA provide a useful model to study changes in chondrocyte biology in response to abnormal environment of the OA joint.

Characterization: Positive for aggrecan after differentiation.

HC-OA have been used to demonstrate that THF-alpha activates transcription of ADAMTS-4, a metalloproteinase implicated in cartilage degradation in OA, via p38-MAPK-dependent mechanism (Xue, 2013).

細胞株の由来

Cartilage

アプリケーション

model for studying chondrocyte biology in an osteoarthritis joint

構成

Basal Medium containing 10% FBS & 10% DMSO

調製ノート

  • 1st passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
  • Can be cultured at least 10 doublings

継代と培養方法

Please refer to the HC-OA Culture Protocol.

免責事項

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

保管分類コード

11 - Combustible Solids

WGK

WGK 3

引火点(°F)

Not applicable

引火点(℃)

Not applicable


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

402OA-05A:


試験成績書(COA)

製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Hongfei Zhang et al.
Clinical rheumatology, 39(11), 3473-3478 (2020-05-11)
LncRNA CTBP1-AS2 has been reported to be involved in type 2 diabetes and cardiomyocyte hypertrophy, while its roles in other human diseases are unknown. Our preliminary deep sequencing analysis showed altered expression of CTBP1-AS2 in osteoarthritis (OA). In addition, CTBP1-AS2
Xiangxiang Sun et al.
International journal of biological sciences, 16(6), 994-1009 (2020-03-07)
Osteoarthritis (OA) is a common type of arthritis. Chronic inflammation is an important contributor to the pathogenesis of OA. The maturation and secretion of proinflammatory cytokines are controlled by inflammasomes, especially NLRP1 (NLR Family Pyrin Domain Containing 1) and NLRP3.

プロトコル

Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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