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由来生物
mouse (Engelbreth-Holm-Swarm (EHS) mouse tumor)
無菌性
sterile
アッセイ
≥90% (SDS-PAGE)
濃度
6 mg/mL
テクニック
cell culture | mammalian: suitable
溶解性
water: miscible
輸送温度
dry ice
保管温度
−70°C
詳細
3-D Culture Matrix™ Mouse Laminin I may be used as a gel on which to grow cells, or as a media additive alone or in concert with other basement membrane components, to study cellular growth and differentiation in three dimensions in vitro. To offer the most standardized Laminin I for use in 3-D cultures, a special process is employed to provide material at a standard concentration of 6 mg/ml (by absorbance and extinction coefficient).
物理的形状
Solution in Dulbecco′s Modified Eagle′s medium containing 10 μg/ml gentamycin sulfate and no phenol red.
その他情報
Functional Assay:
3-D Culture: Laminin I promotes differentiation of a human epithelial cell line derived from mammary gland (MCF-10A) into acinar structures.
Cell Attachment: Tested for the ability to promote cell attachment and spreading of MG63 human osteosarcoma cells.
3-D Culture: Laminin I promotes differentiation of a human epithelial cell line derived from mammary gland (MCF-10A) into acinar structures.
Cell Attachment: Tested for the ability to promote cell attachment and spreading of MG63 human osteosarcoma cells.
Sterility Testing:
No bacterial or fungal growth detected after incubation at 37°C for 14 days following USP XXIV Chapter 71 sterility test.No mycoplasma contamination detected by PCR.Endotoxin concentrations = 20 EU/ml by LAL assay.
No bacterial or fungal growth detected after incubation at 37°C for 14 days following USP XXIV Chapter 71 sterility test.No mycoplasma contamination detected by PCR.Endotoxin concentrations = 20 EU/ml by LAL assay.
Storage Conditions and Product Stability:
Product is stable for a minimum of 3 months from date of shipment when stored at -80 °C in a manual defrost freezer. For optimal stability, store at -80 °C in aliquots. Avoid repeated freeze/thaw cycles.
Product is stable for a minimum of 3 months from date of shipment when stored at -80 °C in a manual defrost freezer. For optimal stability, store at -80 °C in aliquots. Avoid repeated freeze/thaw cycles.
法的情報
3-D Culture Matrix is a trademark of Trevigen, Inc.
保管分類コード
12 - Non Combustible Liquids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
3446-005-01:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Daniel Rüdiger et al.
Cell reports, 32(6), 108015-108015 (2020-08-14)
Endothelial tube formation on a reconstituted basement membrane (Matrigel) is a well-established in vitro model for studying the processes of angiogenesis and vasculogenesis. However, to date, the organizing principles that underlie the morphogenesis of this network and that shape the initial
Ruilin Tian et al.
Neuron, 104(2), 239-255 (2019-08-20)
CRISPR/Cas9-based functional genomics have transformed our ability to elucidate mammalian cell biology. However, most previous CRISPR-based screens were conducted in cancer cell lines rather than healthy, differentiated cells. Here, we describe a CRISPR interference (CRISPRi)-based platform for genetic screens in
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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