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主要文書

3015

Transcreener^® AMP²/GMP² FP Assay

Name: Transcreener® AMP2/GMP2 FP Assay
Brand: SIGMA

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About This Item

UNSPSCコード:

41122100

NACRES:

NA.84

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Millipore

18-161

プロテインG、HRP結合体

輸送温度

dry ice

保管温度

−20°C

詳細

The Transcreener^® AMP2/GMP2 FP Assay is a universal biochemical HTS assay for enzymes that produce AMP or GMP, including ubiquitin, SUMO, nucleic acid and protein ligases, phosphodiesterases (PDEs), and synthetases. Enzyme activity is signaled by a decrease in fluorescence polarization as the bound tracer is displaced from the Transcreener^® AMP2/GMP2 Antibody. The assay is a simple single step mix-and-read format enabling the use of unmodified native substrate concentrations of 1 – 1000 μM. The assay provides excellent signal at low substrate conversion, with a Z′ =0.7 and =100 millipolarization shift (mP) under normal reaction conditions.

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