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Key Documents

安全性情報

111M-1

Sigma-Aldrich

CD11c (5D11) Mouse Monoclonal Antibody

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About This Item

UNSPSCコード:
12352203
NACRES:
NA.41

由来生物

mouse

品質水準

100
500

結合体

unconjugated

抗体製品の状態

culture supernatant

抗体製品タイプ

primary antibodies

クローン

5D11, monoclonal

詳細

For In Vitro Diagnostic Use in Select Regions (See Chart)

形状

buffered aqueous solution

化学種の反応性

human

包装

vial of 0.1 mL concentrate (111M-14)
vial of 0.5 mL concentrate (111M-15)
bottle of 1.0 mL predilute (111M-17)
vial of 1.0 mL concentrate (111M-16)
bottle of 7.0 mL predilute (111M-18)

メーカー/製品名

Cell Marque

テクニック

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:20-1:80

アイソタイプ

IgG2a

コントロール

bone marrow, hairy cell leukemia

輸送温度

wet ice

保管温度

2-8°C

視覚化

cytoplasmic

遺伝子情報

human ... ITGAX(3687)

詳細

CD11c is an adhesion receptor of the leukocyte function-associated family of molecules. This cell surface antigen is normally expressed on granulocytes, monocytes, natural killer cells, and small populations of T- and B-lymphocytes. Therefore, the control for antibody validation and verification should be bone marrow and acute inflammatory tissue blocks. Anti-CD11c, clone 5D11, detects a formalin-resistant epitope of CD11c antigen and will now provide a significant change to the way hairy cell leukemia (HCL) can be diagnosed and assessed in FFPE. The tumor cells of the majority of other types of small B-cell lymphoma do not express this CD11c antigen. HCL is a distinctive yet uncommon chronic B-cell lymphoproliferative disorder, predominantly involving the bone marrow and spleen. Bone marrow aspiration and trephine biopsy are necessary for making a definitive diagnosis and treatment of hairy cell leukemia (HCL) although in exceptional cases, the diagnosis of HCL is made by histological analysis of splenic tissue after splenectomy or splenic needle core biopsy. However, aspiration is successful in only approximately 10% of patients. The marrow films obtained may demonstrate the presence of hairy cells but definitive diagnosis usually requires a bone marrow trephine biopsy due to the high frequency of a dry tap on aspiration. When there is difficulty in obtaining a good marrow aspirate, (frequent occurrence of a dry tap), examination of bone marrow trephine histology together with immunohistochemical analysis is the only available method to make a definitive diagnosis of HCL. Confirmation of the nature of the infiltrate can be obtained by immunocytochemistry performed on paraffin sections using antibodies against CD20, T bet, DBA.44 and TRAcP. However, expression of both TRAcP and DBA.44 (CD72) is not specific for HCL. It should also be noted, when undertaking immunohistochemistry to support a diagnosis of HCL in trephine biopsy sections, that up to 50% of cases show nuclear positivity for cyclin D1although confusion with mantle cell lymphoma (MCL) is less likely for an experienced hematopathologist as the cytology and histology of MCL are different from HCL. Korinna et al. in a recent study used clone (5D11) of anti-CD11c to investigate 31 bone marrow trephines with low-level HCL infiltrates and showed that the anti-CD11c was able to detect HCL to a level of 2% of tumor cells in BM biopsies. This indicates that immunohistochemical staining of formalin-fixed, decalcified bone marrow trephine biopsies with anti-CD11c can be used both for early diagnosis of HCL and for detection of residual disease following therapy. It is important to note that the CD11c-positive interstitial macrophages, which were generally more weakly stained than the hairy cells, did not interfere with the identification of the more strongly stained tumor cells. Among malignant lymphomas, CD11c is consistently expressed in HCL, although it is also rarely detected in B-CLL/small lymphocytic lymphoma, and nodal and extranodal marginal zone lymphoma (MZL).

品質


IVD

IVD

IVD

RUO

物理的形状

Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide

調製ノート

Download the IFU specific to your product lot and formatNote: This requires a keycode which can be found on your packaging or product label.

その他情報

For Technical Service please contact: 800-665-7284 or email: service@cellmarque.com

法的情報

Cell Marque is a trademark of Merck KGaA, Darmstadt, Germany

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保管分類コード

12 - Non Combustible Liquids

WGK

WGK 2

引火点(°F)

Not applicable

引火点(℃)

Not applicable


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

111M-11:
111M-16:
111M-14:
111M-15:
111M-13:
111M-18:
111M-16-RUO:
111M-12:
111M-14-RUO:
111M-17-RUO:
111M-15-RUO:
111M-17:
111M-18-RUO:


試験成績書(COA)

製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Philip Th Went et al.
The American journal of surgical pathology, 29(4), 474-478 (2005-03-16)
Because of marrow fibrosis, bone marrow aspirations are often nonconclusive in patients with hairy cell leukemia (HCL). Therefore, histologic examination is important in HCL but often difficult in cases with low numbers of tumor cells. A combined immunohistochemical positivity for
R N Miranda et al.
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 13(12), 1308-1314 (2001-01-06)
Mantle cell lymphoma (MCL) is more aggressive when compared with other lymphomas composed of small, mature B lymphocytes. Cyclin D1 is overexpressed in MCL as a result of the translocation t(11;14)(q13;q32). Cyclin D1 immunohistochemistry in fixed, paraffin-embedded tissue contributes to
Korinna Johrens et al.
Pathobiology : journal of immunopathology, molecular and cellular biology, 75(4), 252-256 (2008-06-27)
The diagnosis of hairy cell leukemia (HCL) and its distinction from other B-cell lymphomas can be difficult in formalin-fixed tissue. This is because the histology is not always classical, and despite having a characteristic phenotype, there are few relevant monoclonal
G Marotta et al.
Leukemia & lymphoma, 37(1-2), 145-149 (2000-03-18)
This study analyzed the expression of the beta2 integrin CD11c in 155 patients with well-characterized B-cell chronic lymphoproliferative disorders: 106 B-cell chronic lymphocytic leukemias (B-CLL), 21 hairy cell leukemias (HCL), 9 B-cell prolymphocytic leukemias (PLL) and 19 low grade non-Hodgkin's
Gail Jones et al.
British journal of haematology, 156(2), 186-195 (2011-11-25)
The British Committee for Standards in Haematology first produced guidelines for the diagnosis and management of hairy cell leukaemia and hairy cell leukaemia variant in 2000. This revision updates those guidelines and covers the areas of diagnosis, treatment and assessment

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