10112302

Shmac 4 cell line

human prostate, Epithelial

• UNSPSCコード: 41106514

特性

• product name: Shmac 4 cell line,
• 由来生物: human prostate
• 成長モード: Adherent
• 核型: Not specified
• 形態: Epithelial
• 製品: See Lang SH, et.al (2006)
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Prostate cancer moderately well differentiated, early stage

DNAプロファイル

STR-PCR Data:

Amelogenin: X,Y
CSF1PO: 12
D13S317: 11
D16S539: 11,14
D5S818: 12,13
D7S820: 7,10
THO1: 7,9
TPOX: 8
vWA: 17,19

培地

Stemline Keratinocyte Medium II (Sigma S0196) + Stemline Keratinocyte Growth Supplement (Sigma S9945) + 2 mM Glutamine + 2% Fetal Bovine Serum (FBS). Addition of 2% serum was found to increase cell viability.

継代と培養方法

Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at approximately 3 x 10⁴ cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37 °C. It is important to inactivate the trypsin with an equal volume of trypsin inhibitor (the culture medium contains too low a serum content to inactivate the trypsin). To remove all traces of trypsin and trypsin inhibitor pellet the cells by centrifugation and resuspend the cells in fresh medium. Media change every second day.

その他情報

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

免責事項

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable