W0625

ライトステイン

≤4% purity (HPLC), powder

• 別名: Wright法エオシンメチレンブルー
• CAS番号: 68988-92-1
• MDL番号: MFCD00082143
• UNSPSCコード: 12171500
• NACRES: NA.47

特性

• product name: ライトステイン, suitable for blood stain
• アッセイ: ≤4% (HPLC)
• 品質水準: 200
• 形状: powder
• 濃度: ≤4% (HPLC)
• テクニック: microbe id | staining: suitable
• 色: dark green
• 溶解性: methanol: 10 mg/10 mL
• 適合性: suitable for blood stain
• アプリケーション: diagnostic assay manufacturing; hematology; histology
• 保管温度: room temp
• InChI: 1S/C20H8Br4O5.C16H18N3S/c21-11-5-9-13(7-3-1-2-4-8(7)20(27)28)10-6-12(22)17(26)15(24)19(10)29-18(9)14(23)16(11)25;1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13/h1-6H,(H2-,25,26,27,28);5-10H,1-4H3/q;+1/p+1
• InChI Key: AXIKDPDWFVPGOD-UHFFFAOYSA-O

詳細

詳細

Wright′s Stain solution is commercially available and is generally used in hematology. Its preparation involves heating methylene blue with sodium bicarbonate and combining the mixture with eosin. It is alkaline, hence, its concentration is continuously changing due to the progressive oxidizing action of the alkali. Therefore, Giemsa′s stain is added to preserve the azurophilic properties of polychrome dyes.

アプリケーション

Wright′s Stain is mainly used for the staining of peripheral blood smears and bone marrow smears.

• It is also used for G-banding and helps in the analysis of poorly spread metaphases.
• It has been used to study the cytogenetics of human hematopoietic cells.
• It has also been employed in the karyotyping of human embryonic stem cells.
• It is also used in the staining of chromosomes.
• It is used for the exudate analysis of white blood cell concentration.

血液染色において、メイグリュンワルド染色よりも優れていると報告されています。

特徴および利点

The purified dyes in Wright′s Stain eliminate inconsistent staining and yield reproducible lot-to-lot chromogenic responses.

調製ノート

ポリクロ-ム(酸化型)メチレンブル-とエオシンYを用いて調製されています。

安全性情報

• ピクトグラム: GHS07
• シグナルワード: Warning
• 危険有害性情報: H302,H319
• 注意書き: P280 - P301 + P312 + P330 - P305 + P351 + P338 - P337 + P313
• 危険有害性の分類: Acute Tox. 4 Oral - Eye Irrit. 2
• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): dust mask type N95 (US), Eyeshields, Gloves