PPB002

Tris Buffered Saline

powder, pH 8.0, pHast Pack^™, with 0.05% Tween^® 20

• 別名: Tris Buffered Saline, with Tween™ 20, pH 8.0, TBS with 0.05% Tween-20, TBS-T, TBST
• UNSPSCコード: 41105323
• NACRES: NA.21

特性

• 製品名: Tris Buffered Saline, with Tween™ 20, pH 8.0, powder, pHast Pack^™
• 製品種目: pHast Pack^™
• フォーム: powder
• 保管条件: (Tightly closed. Dry.)
• 不純物: ≤5 ppm Heavy Metals (lead)
• 色: white
• pH: 7.8—8.2
• 溶解性: soluble, clear to hazy, colorless
• 微量陽イオン: Fe: ≤10 ppm
• その他の活性: DNase, none detected; Endonuclease ; Exonuclease, none detected; NICKase, none detected; Protease, none detected; RNase, none detected
• 保管温度: room temp

詳細

詳細

Tris Buffered Saline with 0.05% Tween^® 20 or TBST has a low concentration of detergent with a slightly basic pH of 8.0. It is a commonly used washing buffer alternative to PBS-T in many immunodetection techniques. It decreases non-specific bindings from the western blot membrane and microtiter plate walls.

アプリケーション

Suitable for use as a wash buffer in:

• Western blot
• ELISA
• Immunohistochemistry

特徴および利点

• Ready-to-use buffer requires no measurments or pH adjustments
• Saves time, money and effort to get your assays started fast
• Physical, chemical, and biological tests for quality: free of DNase, RNase, Protease, and Nickase
• Qualified for use in gel electrophoreis and other molecular biology assays

包装

Foil pouches

再構成

Contents of one pouch, when dissolved in 500 mL of distilled or deionized water, will yield a 1× solution containing 50 mM Tris, 138 mM NaCl, 2.7 mM KCl; 0.05%Tween 20 (w/v), pH 8.0 at 25 °C. Certified to be DNAse, RNAse, Protease, and Nickase free.

保管および安定性

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

その他情報

For additional information on our range of Biochemicals, please complete this form.

法的情報

TWEEN is a registered trademark of Croda International PLC

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable