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Merck

KK4752

Roche

KAPA PROBE FAST One-Step

Universal

別名:

RT-qPCR, probe qPCR, qPCR

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About This Item

UNSPSCコード:
41106300
NACRES:
NA.55

品質水準

使用法

sufficient for 500 reactions

特徴

dNTPs included: no
hotstart: no

包装

pkg of 500 RXNS

メーカー/製品名

Roche

テクニック

RT-qPCR: suitable

入力

purified DNA

検出方法

probe-based

保管温度

−20°C

詳細

KAPA PROBE FAST One-Step qRT-PCR Master Mix (2X) Universal Kit is a sensitive and convenient solution for real-time PCR using RNA as template. The kit is designed for high-throughput, fast-cycling, one-step RNA quantification using sequence-specific fluorogenic probes. It is compatible with all fluorogenic probe-based technologies, including hybridization probes (e.g., FRET), hydrolysis probes (e.g. TaqMan®) and displacement probes (e.g., molecular beacons)

アプリケーション

KAPA PROBE FAST One-Step qRT-PCR Master Mix (2X) Universal has been used for:
  • Gene expression analysis
  • SNP/mutation analysis
  • Gene knockdown validation
  • RT-PCR

特徴および利点

Quick Notes:
  • The kit is suitable for all fluorogenic probe-based technologies, including hybridization probes (e.g., FRET), hydrolysis probes (e.g., TaqMan) and displacement probes (e.g., molecular beacons).
  • Use only gene-specific primers for one-step qRT-PCR.
  • Optimal cDNA synthesis is achieved at 42°C for 5 min.
  • 3 min at 95°C is sufficient for RT inactivation and DNA polymerase activation.
  • Do not exceed 25 μL reaction volumes

包装

Kit includes qPCR Master Mix (2X), KAPA RT Mix (50X), dUTP (10 mM), ROX High Reference Dye (50X) and ROX Low Reference Dye (50X)

品質

All kit components are subjected to stringent functional quality control, are free of detectable contaminating exo- and endonuclease activity, and meet strict requirements with respect to DNA contamination.

調製ノート

Always ensure that components have been fully thawed and thoroughly mixed before use. The KAPA PROBE FAST qPCR Master Mix may not freeze solidly, even when stored at -20 °C. The KAPA RT Mix is temperature-sensitive, and should be stored at -20 °C and kept on ice during use. ROX reference dye is light-sensitive. Exposure to direct light for an extended period of time will result in loss of fluorescent signal intensity.

その他情報

For Research Use Only. Not for use in diagnostic procedures.

法的情報

TaqMan is a registered trademark of Roche Molecular Systems, Inc.

保管分類コード

12 - Non Combustible Liquids

WGK

WGK 1

引火点(°F)

does not flash

引火点(℃)

does not flash


試験成績書(COA)

製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Simultaneous diagnosis of Cetacean morbillivirus infection in dolphins stranded in the Spanish Mediterranean sea in 2011 using a novel Universal Probe Library (UPL) RT-PCR assay.
Rubio-Guerri C, et al.
Veterinary Microbiology, 165(1-2), 109-114 (2013)

資料

An overview of directed evolution and the methods for generating proteins with optimized or entirely new functions.

プロトコル

Coronavirus qPCR Primer and probe sets for the detection of SARS-CoV-2 (Corona Virus), with additional real-time RT-PCR, RT-qPCR and supporting reagents available.

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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