DTTP-RO

dTTP

PCR Grade, sodium salt

• 別名: チミジン 5′-三リン酸 ナトリウム塩 溶液, TTP
• CAS番号: 18423-43-3
• MDL番号: MFCD00080338
• UNSPSCコード: 12352302
• PubChem Substance ID: 329798929

特性

• 品質水準: 100
• アッセイ: 99% (dNTP)
• フォーム: solution
• 使用法: sufficient for 125,000 standard reactions (03732711001); sufficient for 31,250 standard reactions (11969048001); sufficient for 6,250 standard reactions (11934546001)
• 包装: pkg of 1,250 μL (11969048001 [100 mM]); pkg of 4 × 1,250 μL (03732711001 [4x100 mM]); pkg of 250 μL (11934546001 [100 mM])
• メーカー／製品名: Roche
• 濃度: <0.9% (dNDP); 100 mM
• 保管温度: −20°C
• SMILES記法: [Na].CC1=CN(C2CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O2)C(=O)NC1=O
• InChI: 1S/C10H17N2O14P3.Na.H/c1-5-3-12(10(15)11-9(5)14)8-2-6(13)7(24-8)4-23-28(19,20)26-29(21,22)25-27(16,17)18;;/h3,6-8,13H,2,4H2,1H3,(H,19,20)(H,21,22)(H,11,14,15)(H2,16,17,18);;
• InChI Key: KAELIXTVSANDSE-UHFFFAOYSA-N

詳細

詳細

PCR Grade nucleotides from Roche are specially manufactured and purified to the highest possible chemical purity. PCR Grade nucleotides contain at least 99% of the relevant deoxynucleoside-triphosphate (dNTP) and less than 0.9% deoxynucleoside-diphosphate (dNDP).

Thymidine 5′-triphosphate sodium salt solution is used in DNA synthesis reactions such as PCR, DNA sequencing, cDNA synthesis and molecular cloning techniques.

dTTP, PCR Grade, is a 100 mM clear, colorless solution of the sodium salt (pH 8.3).

アプリケーション

dTTP, PCR Grade, is suitable for many applications where high-quality reagents are required. Such procedures include reverse transcription (RT), polymerase chain reaction (PCR), RT-PCR, DNA labeling reactions, and sequencing/cycle sequencing analysis.

品質

Function tested in RT-PCR. Tested for absence of contaminating deoxyribonucleases, ribonucleases and nicking activities according to the current Quality Control procedures.

物理的形状

solution of dTTP in water (pH 8.3).

調製ノート

Working solution: We recommend to prepare a deoxynucleotide mix containing dATP, dCTP, dGTP, dTTP (10 mM, each); (e.g., for the preparation of 100 μl nucleotide mix add 10 μl of dATP, dCTP, dGTP, dTTP (each) to 60 μl Water, PCR Grade).

その他情報

For general laboratory use.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): does not flash
• 引火点(℃): does not flash