SCT235

BioTracker^™ TAS2 Proteasome Activity Live Cell Probe

Human

• 別名: BioTracker^™ TAS2 Proteasome Activity Live Cell Probe, proteasome activity live cell probe, proteasome activity probe
• UNSPSCコード: 41106514
• NACRES: NA.81

特性

• product name: BioTracker^™ TAS2 Proteasome Activity Live Cell Probe,
• 由来生物: human
• 品質水準: 100
• 形状: lyophilized solid
• 包装: vial of 500 μg
• メーカー／製品名: Millipore
• テクニック: activity assay: suitable; cell culture | stem cell: suitable; flow cytometry: suitable
• 輸送温度: dry ice
• 保管温度: −20°C

詳細

詳細

Existing proteasome activity-based fluorescent probes generally suffer from poor fluorescence and problems with selectivity.
TAS2 is a substrate-based fluorescent probe intended to be cleaved and released by the eukaryotic 20S core particle subunit of the proteasome to monitor proteasome activity. TAS2 demonstrates keen selectivity and resistance to nonspecific cleavage by proteases in human serum. The BioTracker^™ TAS2 Proteasome Activity Probe can be used effectively in applications such as live cell assays, flow cytometry, or live-cell fluorescent microscopy.

MW=1407.12 g/mol
Probe is soluble in DMSO. Working final concentration of probe was 5 - 30 uM in appropriate growth media for 90 minutes.

SPECTRAL PROPERTIES

Excitation max λ: 496 nm
Emission max λ: 524 nm
Imaging was performed with a 488 nm excitation laser and emission window of 515-555 nm.

Zerfas BL, Coleman RW, Salazar-Chaparro AF, Macatangay NJ, Trader DJ. 2020. Fluorescent Probes with Unnatural Amino Acids to Monitor Proteasome Activity in Real-Time. ACS Chemical Biology. 15(9):2588–2596. doi:https://doi.org/10.1021/acschembio.0c00634.

アプリケーション

Purity: > 98% confirmed by HPLC. Structure confirmed by HNMR, LC-MS, and elemental analysis.

特徴および利点

The BioTracker TAS2 Live Cell Probe exhibits strong fluorescence and is highly specific for proteasome activity in mammalian cells.

ターゲットの説明

The proteasome, an elaborate protein complex that controls protein levels and important cellular pathways, is critical to eukaryotic cell function and homeostasis. The proteasome functions to degrade proteins that are damaged or no longer needed. The cell selectively shuttles these proteins to be broken into smaller peptide units that can be recycled and repurposed for other essential needs of the cell. The eukaryotic 26S proteasome is made up of two subunits, the 19S regulatory subunit and 20S core subunit. The proteasome acts by tagging proteins intended for degradation with a chain of ubiquitin which is recognized by the 19S regulatory subunit. After recognition, ubiquitin monomers are removed, and the remaining peptides are hydrolyzed by the 20S core subunit.
Improper proteasome function has been associated with a number of diseases including a variety of cancers. This makes the proteasome a high-interest target for further study and potential therapeutic development.

物理的形状

Pale yellow solid.

保管および安定性

Store BioTracker^™ TAS2 Proteasome Activity Live Cell Probe at -20°C, desiccated and protected from lightNote: Centrifuge vial briefly to collect contents at bottom of vial before opening.

その他情報

Live cell probe for detection of proteasome activity by fluorescence microscopy or flow cytometry. SCT235 is a valuable reagent for research of the function of proteasomes in health and in diseases, including cancer.

法的情報

BioTracker is a trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• ピクトグラム: GHS08
• シグナルワード: Danger
• 危険有害性情報: H334
• 注意書き: P261 - P284 - P501
• 危険有害性の分類: Resp. Sens. 1
• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable