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品質水準
テクニック
nucleic acid detection: suitable (RNA)
詳細
Simplicon is a novel system to effect immediate high sustained protein expression of the target gene(s) without the risk of integration. The technology employs a self-replicating RNA based on the Venezuelan equine encephalitis (VEE) genome1. The Simplicon RNA contains only genes encoding the VEE RNA replication machinery while the structural proteins that are required to make an infectious particle have been completely removed and replaced with the transgene(s) of interest. Because Simplicon is a synthetic RNA, there is no risk of the transgene(s) integrating into the transfected cell’s genome. Moreover, the self-replicating nature of the Simplicon means that the target protein is continuously expressed in the presence of B18R protein and the selective agent, puromycin. The Simplicon technology has been successfully utilized for efficient human iPSC generation through the sustained expression of critical reprogramming factors, OKSG and OKSG-cMyc1.
The Simplicon TagGFP2 RNA Kit was developed to enable the evaluation of the Simplicon expression system in targeted cell(s) before ordering a custom Simplicon RNA. Simplicon TagGFP2 RNA Kit may be used to determine optimal transfection conditions to express the self-replicating RNA in hard-to transfect somatic or primary cells. Simplicon TagGFP2 expresses an improved variant of the Aequorea macrodactyla GFP-like protein. TagGFP2 exhibits bright green fluorescence comparable to that of EGFP, with excitation/emission maxima at 483 and 506 nm, respectively.
The Simplicon TagGFP2 RNA Kit was developed to enable the evaluation of the Simplicon expression system in targeted cell(s) before ordering a custom Simplicon RNA. Simplicon TagGFP2 RNA Kit may be used to determine optimal transfection conditions to express the self-replicating RNA in hard-to transfect somatic or primary cells. Simplicon TagGFP2 expresses an improved variant of the Aequorea macrodactyla GFP-like protein. TagGFP2 exhibits bright green fluorescence comparable to that of EGFP, with excitation/emission maxima at 483 and 506 nm, respectively.
アプリケーション
The Simplicon TagGFP2 RNA Kit was developed to enable the evaluation of the Simplicon expression system in targeted cell(s) before ordering a custom Simplicon RNA. Simplicon TagGFP2 RNA Kit may be used to determine optimal transfection conditions to express the self-replicating RNA in hard-to transfect somatic or primary cells.
構成
1. TagGFP2 RNA: (Part No. CS222751) One (1) vial containing 10 µL of RNA (1 ug/uL). Store at -80°C.
2. B18R RNA: (Part No. CS224516) One (1) vial containing 10 µL of RNA (1 ug/uL). Store at -80°C.
2. B18R RNA: (Part No. CS224516) One (1) vial containing 10 µL of RNA (1 ug/uL). Store at -80°C.
品質
• Discrete RNA band on RNA gel of appropriate size.
• GFP-positive cells observed one day after transfection.
• 40-60% GFP-positive BJ human fibroblasts with FACS analysis.
• GFP-positive cells observed one day after transfection.
• 40-60% GFP-positive BJ human fibroblasts with FACS analysis.
その他情報
Concentration: Please refer to lot specific datasheet.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
保管分類コード
12 - Non Combustible Liquids
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
SCR713:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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