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詳細
Accutase is a cell detachment solution of proteolytic and collagenolytic enzymes that is useful for the routine detachment of cells from standard tissue culture plasticware and adhesion coated plasticware, including SmartPlastic. Accutase is direct replacement for trypsin cell detachment solution and does not require enzyme inactivation with serum. Accutase does not contain mammalian or bacterial derived products.
AccutaseXL is a lyophilized form of Accutase with extended stability. AccutaseXL, once reconstituted, will make 500ml of complete 1X Accutase solution. Note: read detailed reconstitution protocol found in the product datasheet before using this product.
AccutaseXL is a lyophilized form of Accutase with extended stability. AccutaseXL, once reconstituted, will make 500ml of complete 1X Accutase solution. Note: read detailed reconstitution protocol found in the product datasheet before using this product.
Product Source: Accutase is derived from a non-mammalian (invertebrate) source.
アプリケーション
Accutase has been shown to be effective on a wide variety of cell types including: fibroblasts, keratinocytes, vascular endothelial cells, hepatocytes, vascular smooth muscle cells, hepatocyte progenitors, primary chick embryo neuronal cells, bone marrow stem cells, adherent CHO and BHK cells, macrophages, 293 cells, L929 cells, immortalized mouse testicular germ cells, 3T3, Vero, COS, HeLa, NT2, MG63, M24 and A375 metastatic melanoma, gliomas U251 and D54, HT1080 fibrosarcoma cells, and Sf9 insect cells.
品質
pH: 6.8-7.8
Activity Assay: 500-20 units/ml (1 unit will release 1 µmole pNA from SUCR-pNA per minute at 37˚C)
Functional Assay: Cell detachment assay
Activity Assay: 500-20 units/ml (1 unit will release 1 µmole pNA from SUCR-pNA per minute at 37˚C)
Functional Assay: Cell detachment assay
物理的形状
1X Accutase enzymes in Dulbecco’s PBS (0.2 g/L KCl, 0.2 g/L KH2PO4, 8 g/L NaCl, and 1.15 g/L Na2HPO4) containing 0.5 mM EDTA4Na and 3 mg/L Phenol Red.
保管および安定性
Store lyophilized AccutaseXL at -20°C for up to 4 months from date of receipt.
Once reconstituted in water, liquid Accutase can be aliquoted and frozen at -20°C for 2 years or stored for 2 months at 4°C. It is recommended to thaw liquid Accutase at 4°C overnight or in a bath of cool water.
Once reconstituted in water, liquid Accutase can be aliquoted and frozen at -20°C for 2 years or stored for 2 months at 4°C. It is recommended to thaw liquid Accutase at 4°C overnight or in a bath of cool water.
法的情報
Accutase is a registered trademark of Innovative Cell Technologies, Inc.
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
シグナルワード
Warning
危険有害性情報
危険有害性の分類
Skin Sens. 1
保管分類コード
10 - Combustible liquids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
SCR104:
試験成績書（COA）
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