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由来生物
mouse
品質水準
包装
vial of ≥1 x 10^6 vial (viable cells per vial)
メーカー／製品名
Millipore
テクニック
cell culture | mammalian: suitable
輸送温度
liquid nitrogen
保管温度
−196°C
アプリケーション
- Cells are verified to be of mouse origin and negative for human, rat, Chinese hamster, Golden Syrian hamster, and non-human primate interspecies contamination, as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services
- Cells tested negative for infectious diseases against a Mouse Essential CLEAR panel by Charles River Animal Diagnostic Services.
- Cells tested negative for mycoplasma.
特徴および利点
The MIN6 cell line is a long-term stable mouse pancreatic beta-cell line that retains characteristics of mature β-cells and can be used to analyze insulin secretion/sensitivity in diabetes research.
ターゲットの説明
The Mouse Insulinoma Cell Line (MIN6), was derived from an insulinoma tissue that developed in a transgenic mouse in which the expression of the Simian Virus 40 Large T antigen was driven by the human insulin promoter.1,2,3 In comparison to the primary pancreatic b-cells, MIN6 thrives reliably and far more robustly in cell culture and yet retains the important characteristics of the b-cells, such as the exclusive expression of the so-called “liver-type” glucose transporter (Glut2) and their ability to modulate the insulin secretion in response to the changing glucose concentration in the growth medium, and it has been a staple strain in diabetes research since its establishment in 1990.
Additionally, the expression of the major histocompatibility complex I (MHC I) is lacking in MIN6, as in the non-diabetic b-cells, but can be induced by pro-inflammatory cytokines,1,4,5 which would render them susceptible to T-cell mediated cytotoxicity. This closely mimics the behavior of the b-cells isolated from diabetic tissues, where chronic inflammation is commonly observed, and it has made MIN6 an attractive cell line model for investigating the mechanism of the b-cells loss due to their autoimmune destruction.
References
1. Miyazaki J, Araki K, Yamato E, Ikegami H, Asano T, Shibasaki Y, Oka Y, Yamamura K. 1990. Establishment of a pancreatic beta cell line that retains glucose-inducible insulin secretion: special reference to expression of glucose transporter isoforms. Endocrinology. 27(1):126-32.
2. Sarvetnick N, Liggitt D, Pitts SL, Hansen SE, Stewart TA. 1988. Insulin-dependent diabetes mellitus induced in transgenic mice by ectopic expression of class II MHC and interferon-gamma. Cell. 52(5):773-82. Pytynia KB, Dahlstrom KR, Sturgis EM. 2014. Epidemiology of HPV-associated oropharyngeal cancer. Oral Oncol. 50(5): 380-6.
3. Ullrich A, Dull TJ, Gray A, Philips JA 3rd, Peter S. 1982. Variation in the sequence and modification state of the human insulin gene flanking regions. Nucleic Acids Res. 10(7):2225-40.
4. Jiang H, Li Y, Shen M, Liang Y, Qian Y, Dai H, Xu K, Xu X, Lv H, Zhang J, Yang T, Fu Q. 2022. Interferon-α promotes MHC I antigen presentation of islet α cells through STAT1-IRF7 pathway in type 1 diabetes. Immunology. 166(2):210-221.
5. Javeed N, Her TK, Brown MR, Vanderboom P, Rakshit K, Egan AM, Vella A, Lanza I, Matveyenko AV. 2021. Pro-inflammatory α cell small extracellular vesicles induce α cell failure through activation of the CXCL10/CXCR3 axis in diabetes. Cell Rep. 36(8):109613.
Additionally, the expression of the major histocompatibility complex I (MHC I) is lacking in MIN6, as in the non-diabetic b-cells, but can be induced by pro-inflammatory cytokines,1,4,5 which would render them susceptible to T-cell mediated cytotoxicity. This closely mimics the behavior of the b-cells isolated from diabetic tissues, where chronic inflammation is commonly observed, and it has made MIN6 an attractive cell line model for investigating the mechanism of the b-cells loss due to their autoimmune destruction.
References
1. Miyazaki J, Araki K, Yamato E, Ikegami H, Asano T, Shibasaki Y, Oka Y, Yamamura K. 1990. Establishment of a pancreatic beta cell line that retains glucose-inducible insulin secretion: special reference to expression of glucose transporter isoforms. Endocrinology. 27(1):126-32.
2. Sarvetnick N, Liggitt D, Pitts SL, Hansen SE, Stewart TA. 1988. Insulin-dependent diabetes mellitus induced in transgenic mice by ectopic expression of class II MHC and interferon-gamma. Cell. 52(5):773-82. Pytynia KB, Dahlstrom KR, Sturgis EM. 2014. Epidemiology of HPV-associated oropharyngeal cancer. Oral Oncol. 50(5): 380-6.
3. Ullrich A, Dull TJ, Gray A, Philips JA 3rd, Peter S. 1982. Variation in the sequence and modification state of the human insulin gene flanking regions. Nucleic Acids Res. 10(7):2225-40.
4. Jiang H, Li Y, Shen M, Liang Y, Qian Y, Dai H, Xu K, Xu X, Lv H, Zhang J, Yang T, Fu Q. 2022. Interferon-α promotes MHC I antigen presentation of islet α cells through STAT1-IRF7 pathway in type 1 diabetes. Immunology. 166(2):210-221.
5. Javeed N, Her TK, Brown MR, Vanderboom P, Rakshit K, Egan AM, Vella A, Lanza I, Matveyenko AV. 2021. Pro-inflammatory α cell small extracellular vesicles induce α cell failure through activation of the CXCL10/CXCR3 axis in diabetes. Cell Rep. 36(8):109613.
保管および安定性
The cells should be stored in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
その他情報
This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
保管分類コード
12 - Non Combustible Liquids
WGK
WGK 2
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
労働安全衛生法名称等を表示すべき危険物及び有害物
名称等を表示すべき危険物及び有害物
労働安全衛生法名称等を通知すべき危険物及び有害物
名称等を通知すべき危険物及び有害物
Jan Code
SCC623:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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