QIA127

Rapid Cell Proliferation Kit

• 別名: Proliferation assay kit
• UNSPSCコード: 12352207
• NACRES: NA.84

特性

• 使用法: sufficient for 500 tests
• 品質水準: 100
• 包装: pkg of 1 96-well plate(s)
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze; avoid repeated freeze/thaw cycles; protect from light
• 入力: sample type cultured cells (1,000-50,000 cells)
• 検出方法: colorimetric
• 輸送温度: wet ice
• 保管温度: −20°C

詳細

詳細

Assay measures the increased activity of cellular mitochondrial dehydrogenases that can cleave the tetrazolium dye WST-1 to formazan. The formazan formation is then quantified by measuring the change in absorbance at 450 nm in a microplate reader. The activity of mitochondrial dehydrogenases is proportional to cell number. No washing, harvesting, or solubilization steps are required.

特徴および利点

Sensitivity: 1000 cells (adherent); 2500 cells (nonadherent)

構成

WST-1, 1-Methoxy PMS, and a user protocol.

警告

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

規格

Assay Time: 0.5 - 4 h

原理

The Calbiochem Rapid Cell Proliferation Kit is designed to determine the number of viable cells in proliferation, or cytotoxicity studies, based on the cleavage of the tetrazolium salt WST-1 by mitochondrial dehydrogenases in viable cells. The assay can be used to measure cell proliferation in response to growth factors or cytokines, or to assess cytotoxic compounds.

調製ノート

• WST-1 Labeling Mixture: Thaw the EMS solution at room temperature or in a 37°C water bath. Add the entire contents of this solution to the bottle containing the WST-1 reagent. The mixed solution can be stored at 4°C for several weeks. For long-term storage (several months) aliquot and store at -20°C. Protect from light.

その他情報

Ishiyama, M., et al. 1995. In Vitro Toxicology8, 187.
Liu, S.O., et al 1995. Nat. Med.1, 267.
Ishiyama, M., et al. 1993. Chem. Pharm. Bull.41, 1118.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 10 - Combustible liquids