OP17L

Anti-c-Fos (Ab-1) Mouse mAb (2G9C3)

lyophilized, clone 2G9C3, Calbiochem^®

• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: 2G9C3, monoclonal
• 形状: lyophilized
• 含まれません: preservative
• 化学種の反応性: rat, human, mouse
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze
• アイソタイプ: IgG2a
• 輸送温度: ambient
• 保管温度: 2-8°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... FOS(2353)

詳細

詳細

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with AG.8653X mouse myeloma cells. Recognizes the ~55 kDa c-fos and the ~62 kDa v-fos proteins.

Recognizes both the ~55 kDa c-Fos and ~62 kDa v-Fos proteins in HeLa cells. Does not cross-react with the ~39 kDa Jun protein.

This Anti-c-Fos (Ab-1) Mouse mAb (2G9C3) is validated for use in Immunoblotting, Immunocytochemistry, Paraffin Sections for the detection of c-Fos (Ab-1).

免疫原

Human

a synthetic peptide (KVEQLSPEEEEKRRIRRERNKMAAA) corresponding to amino acids 128-152 of human c-Fos

アプリケーション

Immunoblotting (2.5-5 µg/ml)

Immunocytochemistry (2.5-5 µg/ml)

Paraffin Sections (not recommended)

警告

Toxicity: Standard Handling (A)

物理的形状

Lyophilized from a volatile buffer, 200 µg BSA.

再構成

Reconstitute in sterile PBS, PH 7.4, or sterile Tris-saline (20 mM Tris containing 150 mM NaCl), pH 7.4, to a final concentration of 200 µg/ml. To achieve complete solubilization, reconstitute at (4°C) with 0.1% sodium azide or aliqout and freeze (-20°C) for long-term storage. Note: sodium azide is cytotoxic.

アナリシスノート

Positive Control
HeLa cells

その他情報

Jensen, D.E., et al. 1991. Oncogene6, 1219.
Reiss, M., et al. 1990. Cancer Res.50, 6641.
RouscherIII, F.J., et al. 1988. Science240, 1010.
Sassone-Corsi, P., et al. 1988. Cell54, 553.
Giardina, S.L., et al. 1987. Anal Biochem. 161, 109.
Verma, I.M., et al. 1987. Cancer Res.49, 29.
Verma, I.M., et al. 1987. Cell51, 513.
Muller, R. 1986. Viochem. Biophys. Acta.823, 207.
Verma, P. 1986. Trends Genet.2, 93.
Greenberg, M., et al. 1984. Nature (London)311, 433.

Recognizes both cellular and viral forms of fos. Does not react with the ~39 kDa jun protein. Antibody should be titrated for optimal results in individual systems.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 1