MABT418

Anti-GPVI Antibody, clone 8A2, Detection Antibody

clone 8A2, from rat

• 別名: Platelet glycoprotein VI, GPVI, Glycoprotein 6
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rat
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: 8A2, monoclonal
• 化学種の反応性: human
• テクニック: ELISA: suitable; western blot: suitable
• アイソタイプ: IgG1κ
• NCBIアクセッション番号: NP_001242946
• UniProtアクセッション番号: Q9HCN6
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... GP6(51206)

詳細

詳細

Glycoprotein VI (GPVI), or glycoprotein 6 (GP6), is a member of the immunoglobulin gene superfamily, closely related to Fc receptor gamma chain (FcRgamma) and natural killer receptors. GPVI is a collagen receptor involved in collagen-induced platelet adhesion and activation, and may contribute to arterial and venous thrombus formation. GPVI is a membrane glycoprotein that plays a crucial role in platelet procoagulant activity and subsequent thrombin and fibrin formation. GPVI is expressed in megakaryocytes and platelets. The signaling pathway of GPVI involves the FcRgamma, the Src kinases (likely Fyn/Lyn), the adapter protein LAT and leads to the activation of phospholipase C gamma2. It has been shown that bleeding disorders such as Bleeding Disorder Platelet-Type 11 (BDPLT11), a mild to moderate bleeding disorder caused by defective platelet activation and aggregation in response to collagen, is caused by defects in GPVI.

免疫原

Recombinant protein corresponding to human GPVI.

アプリケーション

Anti-GPVI Antibody, clone 8A2, Detection Antibody is an antibody against GPVI for use in western blotting & ELISA.

ELISA Assay: A representative lot from an independent laboratory detected recombinant GPVI in a bead-based sandwich immunoassay (Bigalke, B., et al. (2011). Clin Chem. 57(6):898-904.). This antibody should be used in conjunction with Cat. No. MABT406, Anti-GPVI, clone 8E9, Capture Antibody, as an ELISA detection and capture antibody pair.

品質

Evaluated by Western Blotting in recombinant human GPVI glycoprotein.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected recombinant human GPVI glycoprotein.

ターゲットの説明

~50 kDa observed. The calculated molecular weight of this protein is 37 kDa However, a recombinant protein was observed at 50-55 kDa in Western Blotting.

関連事項

Replaces: MABT473

物理的形状

Format: Purified

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable