MABT1547

Anti-CFAP206 Antibody, clone ORF2-2A7

• 別名: Cilia- and flagella-associated protein 206
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: rat
• 品質水準: 200
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: ORF2-2A7, monoclonal
• 分子量: calculated mol wt 70.95 kDa; observed mol wt ~70 kDa
• 精製方法: using protein G
• 化学種の反応性: mouse
• 包装: antibody small pack of 100 μL
• テクニック: western blot: suitable
• アイソタイプ: IgG2aκ
• エピトープ配列: C-terminal
• タンパク質IDアクセッション番号: NP_001333991
• UniProtアクセッション番号: Q6PE87
• 保管温度: 2-8°C
• 遺伝子情報: mouse ... Cfap206(69329)

詳細

特異性

Clone ORF2-2A7 is a rat monoclonal antibody that detects Cilia- and flagella-associated protein 206 (CFAP206). It targets an epitope within 13 amino acids from the C-terminal region.

免疫原

A linear peptide corresponding to 13 amino acids from the C-terminal region of mouse Cilia- and flagella-associated protein 206 (CFAP206).

アプリケーション

Quality Control Testing

Evaluated by Western Blotting in Mouse testis tissue extract.

Western Blotting Analysis: A 1:500 dilution of this antibody detected CFAP206 in Mouse testis tissue extract.

Tested Applications

Western Blotting Analysis: A representative lot detected CFAP206 in Western Blotting application. (Beckers, A., et al. (2020). Development. 147(21):dev188052).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

ターゲットの説明

Cilia- and flagella-associated protein 206 (UniProt: Q6PE87; also known as CFAP206) is encoded by the Cfap206 gene (Gene ID: 69329) in murine species. CFAP206 is an evolutionarily conserved protein that lacks known motifs or domains, except for a unique 280 amino acid domain with conserved motifs GFC and GIL. It localizes to the basal body/ centrosome and to cilia, and its expression correlates with and depends on Foxj1 expression. In vertebrates it plays a role during motile cilia assembly, and its deficiency can lead to abnormal ciliary beating and decreased sperm motility. It is reported to be a potential component of the nexin-dynein regulatory complex and interacts with radial spoke protein 3. Two isoforms of CFAP206 have been described that are produced by alternative spicing. Isoform 1 is expressed in the sperm, oviduct, lung, nasal cavity, brain ependyma, and choroid plexus. This isoform is shown to be essential for sperm motility and is involved in the regulation of the beating frequency of motile cilia on the epithelial cells of the respiratory tract and it is required for the establishment of radial spokes in sperm flagella. Isoform 2 that is expressed in the sperm, oviduct, lung, nasal cavity, brain ependyma, and choroid plexus is also required for sperm motility and is involved in the regulation of the beating frequency of motile cilia on the epithelial cells of the respiratory tract. Clone ORF2-2A7 specifically detects isoform 1 and detects a sequence within 13 amino acids from the C-terminal region, which is missing in isoform 2. (Ref.: Beckers, A., et al. (2020). Development. 147(21); dev188052).

物理的形状

Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

再構成

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

保管および安定性

Recommended storage: +2°C to +8°C.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable