MABS528

Anti-HGPRT Antibody, clone 13H11.1

clone 13H11.1, from mouse

• 別名: Hypoxanthine-guanine phosphoribosyltransferase, HGPRT, HGPRTase
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: 13H11.1, monoclonal
• 化学種の反応性: human
• テクニック: western blot: suitable
• アイソタイプ: IgG2bκ
• NCBIアクセッション番号: NP_000185
• UniProtアクセッション番号: P00492
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... HPRT1(3251)

詳細

詳細

Hypoxanthine-guanine phosphoribosyltransferase (EC:2.4.2.8; UniProt P00492; also known as HGPRT, HGPRTase) is encoded by the HPRT1 (also known as HGPRT, HPRT) gene (Gene ID 3251) in human. HPRT is a purine salvage enzyme that catalyzes the condensation of 5-phosphoribosyl 1-pyrophosphate (PRPP) and hypoxanthine or guanine to form inosine 5-monophosphate (IMP) and guanosine 5-monophosphate (GMP), respectively. PRPP is an essential compound for the de novo pathway of purine synthesis as well as the synthesis of pyrimidine compounds. PRPP concentrations in erythrocyte are markedly increased in individuals with HPRT deficiency. Complete HPRT deficiency leads to Lesch–Nyhan disease (LND) characterized by uric acid overproduction, motor dysfunction, cognitive disability, and self-injurious behavior. Partial deficiency of HPRT activity causes Lesch–Nyhan variant (LNV) characterized by hyperuricemia and variable degree of neurological manifestations, depending on the residual enzyme activity.

免疫原

Epitope: Internal

GST-tagged human HGPRT recombinant fragment lacking N- and C-terminal regions.

アプリケーション

Research Category
細胞シグナル伝達

Research Sub Category
神経シグナリング

This Anti-HGPRT, clone 13H11.1 is validated for use in Western Blotting for the detection of HGPRT.

品質

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected HGPRT in 10 µg of HeLa cell lysate.

ターゲットの説明

~25 kDa observed

物理的形状

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable