MABS275

Anti-Gli3 Antibody, clone 5E1

clone 5E1, from mouse

• 別名: Transcriptional activator GLI3, GLI3 form of 190 kDa, GLI3-190, GLI3FL, GLI3 C-terminally truncated form, GLI3 form of 83 kDa, GLI3-83
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• クローン: 5E1, monoclonal
• application: ICC; IHC; WB
• 化学種の反応性: mouse, human
• テクニック: immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• citations: 1

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: 5E1, monoclonal
• 化学種の反応性: mouse, human
• テクニック: immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• アイソタイプ: IgMκ
• NCBIアクセッション番号: NP_000159.3
• UniProtアクセッション番号: P10071
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... GLI3(2737)

詳細

詳細

Gli3 can function as both a transcriptional activator and repressor of the sonic hedgehog (Shh) pathway. The full-length Gli3 form (GLI3FL) becomes an activator (GLI3A) after phosphorylation and nuclear translocation. The C-terminally truncated form (GLI3R), acts as a repressor. Proper balance between the Gli3 activator and the repressor specifies limb digit number and identity during development. In concert with TRPS1, Gli3 plays a role in activating chondrocyte proliferation. Gli3 is expressed in several normal adult tissues, including lung, colon, spleen, placenta, testis, and myometrium. Gli3 defects are associated with Greig cephalo-poly-syndactyly syndrome (GCPS), Pallister-Hall syndrome (PHS), polydactyly postaxial type A1 (PAPA1), polydactyly postaxial type B polydactyly (PAPB), and polydactyly preaxial type 4 (POP4).

特異性

This antibody recognizes the truncated, repressor form and the full length protein of human Gli3.

免疫原

Recombinant protein corresponding to the putative repressive motif of human GLI3.

アプリケーション

Research Category
細胞シグナル伝達

Research Sub Category
発生シグナル伝達

Immunocytochemistry Analysis: A representative lot detected Gli3 in PK-15, COS-1, and TM4 cells cotransfected with recombinant Gli3 (Hunt, R., et al. (2007). Hybridoma. 27(4):231-240.).

Immunohistochemistry Analysis: A representative lot detected Gli3 in mouse embryo cross-sections (Laht, S., et al. (2008). Hybridoma. 27(3):167-174.).

This Gli3 antibody is validated for use in WB, ICC & IHC for the detection of the Gli3 protein.

品質

Evaluated by Western Blotting in human thymus tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected in Gli3 in 10 µg of human thymus tissue lysate.

ターゲットの説明

~75 kDa and ~200 kDa observed. The truncated, repressor form and full length form of this protein has been observed at ~83 kDa and ~190 kDa, respectively (Tanimoto, Y., et al. (2012). J Biol Chem. 287(25):21429-21438.). Uncharacterized bands may be observed at ~160 kDa and below ~50 kDa in some cell lysates.

物理的形状

Format: Purified

Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

アナリシスノート

Control
Human thymus tissue lysate

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable