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由来生物
mouse
品質水準
抗体製品の状態
purified antibody
抗体製品タイプ
primary antibodies
クローン
B9, monoclonal
分子量
calculated mol wt 35.09 kDa
精製方法
using protein G
化学種の反応性
human
包装
antibody small pack of 100 μg
テクニック
ELISA: suitable
immunoprecipitation (IP): suitable
アイソタイプ
IgG3λ
エピトープ配列
C-terminal
タンパク質IDアクセッション番号
UniProtアクセッション番号
保管温度
2-8°C
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... ASGR2(433)
詳細
Asialoglycoprotein receptor 2 (UniProt: P07307; also known as ASGP-R 2, ASGPR 2, C-type lectin domain family 4 member H2, Hepatic lectin H2, HL-2) is encoded by the ASGR2 (also known as CLEC4H2) gene (Gene ID: 433) in human. ASGPR is a type II membrane glycoprotein that contains a cytoplasmic domain (aa 1-58), a transmembrane domain (aa 59-79), and an extracellular domain (aa 80-311). Its C-type lectin domain is localized in amino acids 176-302. ASGPR is expressed exclusively in hepatic parenchymal cells where it serves in clearance of asialoglycoproteins from the plasma. It mediates the endocytosis of plasma glycoproteins to which the terminal sialic acid residue on their complex carbohydrate moieties has been removed. The receptor recognizes terminal galactose and N-acetylgalactosamine units. After ligand binding to the receptor, the resulting complex is internalized and transported to a sorting organelle, where receptor and ligand are disassociated, and the receptor is returned to the cell membrane surface. A soluble form of ASGPR (~ 35 kDa) has also been described and is termed as sH2a that is formed by cleavage in the endoplasmic reticulum of its precursor. Levels of ASGPR sH2a are significantly reduced under conditions of liver cirrhosis and fibrosis. Clone B9 specifically detects the secreted sH2a form of ASGPR in human plasma and in NIH3T3 cells transfected with human ASGPR sH2a. (Ref.: Shenkman, M., et al. (2013). J. Biol. Chem. 288(4); 2167-2178; Benyair, R., et al. (2011). World J. Gastroenterol. 17(48); 5305-5309).
特異性
Clone B9 is a mouse monoclonal antibody that detects Asialoglycoprotein receptor 2 (ASGPR2). It targets an epitope within 12 amino acids from the C-terminal region.
免疫原
KLH-conjugated linear peptide corresponding to 12 amino acids from the C-terminal region of human Asialoglycoprotein receptor 2.
アプリケーション
Quality Control Testing
Evaluated by ELISA with recombinant human ASGPR protein.
ELISA Analysis (ELISA): Various dilutions of this antibody detected recombinant human ASGPR sH2a protein.
Tested Applications
Immunoprecipitation Analysis: A representative lot immunoprecipitated ASGPR sH2a in Immunoprecipitation applications (Benyair, R., et al. (2011). World J Gastroenterol. 17(48):5305-9; Shenkman, M., et al. (2013). J Biol Chem. 288(4):2167-78).
ELISA Analysis: A representative lot detected ASGPR sH2a in ELISA applications (Benyair, R., et al. (2011). World J Gastroenterol. 17(48):5305-9).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by ELISA with recombinant human ASGPR protein.
ELISA Analysis (ELISA): Various dilutions of this antibody detected recombinant human ASGPR sH2a protein.
Tested Applications
Immunoprecipitation Analysis: A representative lot immunoprecipitated ASGPR sH2a in Immunoprecipitation applications (Benyair, R., et al. (2011). World J Gastroenterol. 17(48):5305-9; Shenkman, M., et al. (2013). J Biol Chem. 288(4):2167-78).
ELISA Analysis: A representative lot detected ASGPR sH2a in ELISA applications (Benyair, R., et al. (2011). World J Gastroenterol. 17(48):5305-9).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
物理的形状
Purified mouse monoclonal antibody IgG3 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
再構成
1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
保管および安定性
Stable for 1 year at +2°C to +8°C from date of receipt.
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
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Jan Code
MABS2304-25UG:
MABS2304-100UG:
試験成績書（COA）
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