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由来生物
mouse
品質水準
抗体製品の状態
purified antibody
抗体製品タイプ
primary antibodies
クローン
234-4.2, monoclonal
化学種の反応性
human
化学種の反応性（ホモロジーによる予測）
rat (immunogen homology)
テクニック
immunohistochemistry: suitable
western blot: suitable
アイソタイプ
IgG2aκ
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
wet ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... KRAS(3845)
詳細
GTPase KRas (c-k-ras; K-ras) is a membrane-bound G protein belonging to the family of Ras GTPases. They are activated by the binding of GTP in response to signals from various receptors including the epidermal growth factor receptor (EGFR). Ras GTPases are regulated by guanine nucleotide exchange factors (GEF) and GTPase activating proteins (GAP) and lie upstream of the MAP kinase pathway which controls a range of cellular processes including proliferation and differentiation. Previous studies have suggested that KRas plays an important role in embryonic development, and it may also contribute to the pathogenesis of colorectal cancer via an EGFR-mediated pathway.
免疫原
Recombinant protein corresponding to rat c-K-Ras.
アプリケーション
Research Category
細胞シグナル伝達
細胞シグナル伝達
Research Sub Category
GPCR、cAMP/cGMP及びカルシウムシグナル伝達
GPCR、cAMP/cGMP及びカルシウムシグナル伝達
This Anti-c-K-Ras Antibody, clone 234-4.2 is validated for use in Western Blotting, IHC for the detection of c-K-Ras.
Western Blot Analysis: A representative lot detected c-K-Ras in A431 cell lysate.
Western Blot Analysis: A representative lot from an independent laboratory detected c-K-Ras in primary human
lymphoid leukemia cell lysates, and in normal and HA-dynamin K44A mutant HeLa cell lysates (Omerovic, J., et al. (2008). Oncogene. 27(19):2754-2762.).
Immunohistochemistry Analysis: A representative lot from an independent laboratory detected c-K-Ras in non-small cell lung cancer cells (Volm, M., et al. (2002). Clin Cancer Res. 8(6):1843-1848.).
Western Blot Analysis: A representative lot from an independent laboratory detected c-K-Ras in primary human
lymphoid leukemia cell lysates, and in normal and HA-dynamin K44A mutant HeLa cell lysates (Omerovic, J., et al. (2008). Oncogene. 27(19):2754-2762.).
Immunohistochemistry Analysis: A representative lot from an independent laboratory detected c-K-Ras in non-small cell lung cancer cells (Volm, M., et al. (2002). Clin Cancer Res. 8(6):1843-1848.).
品質
Evaluated by Western Blot in SW-480 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected c-K-Ras in 10 µg of SW-480 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected c-K-Ras in 10 µg of SW-480 cell lysate.
ターゲットの説明
~22 kDa observed
物理的形状
Protein G Purified
Format: Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
保管および安定性
Stable for 1 year at 2-8°C from date of receipt.
アナリシスノート
Control
SW-480 cell lysate
SW-480 cell lysate
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Ras isoform abundance and signalling in human cancer cell lines.
Omerovic, J, et al.
Oncogene, 27, 2754-2762 (2008)
Elaine Lai-Han Leung et al.
International journal of cancer, 145(5), 1334-1345 (2019-02-21)
Oncogenic KRAS is considered a promising target for anti-cancer therapy. However, direct pharmacological strategies targeting KRAS-driven cancers remained unavailable. The prenyl-binding protein PDEδ, a transporter of KRAS, has been identified as a potential target for pharmacological inhibitor by selectively binding
Expression profile of genes in non-small cell lung carcinomas from long-term surviving patients.
Volm, Manfred, et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 8, 1843-1848 (2002)
Enkhtsetseg Munkhbaatar et al.
Nature communications, 11(1), 4527-4527 (2020-09-12)
Evasion of programmed cell death represents a critical form of oncogene addiction in cancer cells. Understanding the molecular mechanisms underpinning cancer cell survival despite the oncogenic stress could provide a molecular basis for potential therapeutic interventions. Here we explore the
Almas Yaqoob et al.
PloS one, 15(5), e0231948-e0231948 (2020-05-06)
In our search for bioactive mushrooms native to British Columbia, we determined that the ethanol extracts from fruiting bodies of the terrestrial polypore Albatrellus flettii had potent anti-cell viability activity. Using bioassay-guided fractionation, mass spectrometry and nuclear magnetic resonance, we
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