MABS1819M

Anti-OTUD3 Antibody, clone 12A6.1

clone 12A6.1, from mouse

• 別名: OTU domain-containing protein 3, EC 3.4.19.12
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.43

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: 12A6.1, monoclonal
• 化学種の反応性: human
• 包装: antibody small pack of 25 μg
• テクニック: western blot: suitable
• アイソタイプ: IgG1κ
• NCBIアクセッション番号: NP_056022
• UniProtアクセッション番号: Q5T2D3
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... OTUD3(23252)

詳細

詳細

OTU domain-containing protein 3 (UniProt: Q5T2D3; also known as EC: 3.4.19.12, OTUD3) is encoded by the OTUD3 (also known as KIAA0459) gene (Gene ID: 23252) in human. OTUD3 is a cytoplasmic deubiquitinating enzyme that hydrolyzes Lys-6- and Lys-11-linked polyubiquitin and is reported to hydrolyze heterotypic (mixed and branched) and homotypic chains. It has an OUT domain (aa 65-189) and a UBA-like domain (aa 230-270). Its specificity is given by the S1′ ubiquitin-binding site within the OTU domain composed of the Cys-, His- and Variable-loops. The UBA-like domain has no effect on ubiquitin hydrolysis. It displays a thiol-dependent ubiquitin-specific protease activity. It is reported to regulate PTEN stability and suppresses tumorigenesis. OTUD3 transgenic mice exhibit higher levels of the PTEN and are shown to be less prone to tumorigenesis. OTUD3 is believed to regulate PTEN in a manner, which is dependent on its DUB activity. The catalytically inactive mutant C76A does not upregulate PTEN and may even have a downregulating effect. It can remove Lys-6, Lys-11, Lys-27, and Lys-48 types of ubiquitin chain on PTEN. (Ref.: Yuan, L., et al (2015). Nat. Cell Biol. 17(9); 1169-1181).

特異性

Clone 12A6.1 detects OTU domain-containing protein 3 in human cells. It iargets an epitope within 115 amino acids from the C-terminal region.

免疫原

GST/His-tagged recombinant fragment corresponding to 115 amino acids from the C-terminal half of human OTU domain-containing protein 3.

アプリケーション

Research Category
細胞シグナル伝達

Anti-OTUD3, clone 12A6.1, Cat. No. MABS1819, is a mouse monoclonal antibody that detects OTUD3 in human cells and has been tested for use in Western Blotting.

品質

Evaluated by Western Blotting in human cerebral cortex tissue lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected OTUD3 in 10 µg of human cerebral cortex tissue lysate.

ターゲットの説明

~48 kDa observed; 45.12 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

物理的形状

Protein G purified

Format: Purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1