MABS1270

Anti-Lipoprotein Lipase/LPL Antibody, clone 4-1a

clone 4-1a, from mouse

• 別名: Lipoprotein lipase, hLPL, LPL
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: 4-1a, monoclonal
• 化学種の反応性: human, mouse, bovine, rat
• テクニック: ELISA: suitable; immunocytochemistry: suitable; immunofluorescence: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• アイソタイプ: IgG2aκ
• NCBIアクセッション番号: NP_000228
• UniProtアクセッション番号: P06858
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... LPL(4023)

詳細

詳細

Lipoprotein lipase (EC 3.1.1.34; UniProt P06858; also known as hLPL, LPL) is encoded by the LPL (also known as LIPD) gene (Gene ID 4023) in human. Lipoprotein lipase (LPL) catalyzes the hydrolysis of triglycerides in plasma lipoproteins. LPL is produced by adipocytes and myocytes and secreted into the interstitial spaces, where it is bound by GPIHBP1 (a glycosylphosphatidylinositol-anchored protein of capillary endothelial cells) and shuttled to the luminal face of capillaries. The GPIHBP1–LPL complex is crucial for the binding of triglyceride-rich lipoproteins (TRLs) to endothelial cells and the subsequent lipolytic processing of TRLs. TRLs bind only the LPL-GPIHBP1 complex, but not GPIHBP1 alone, on the cell surface. A deficiency of either protein results in severe hypertriglyceridemia (chylomicronemia) and impaired delivery of lipid nutrients to parenchymal cells. LPL is produced with a signal peptide sequence (a.a. 1-27), the removal of which yields the mature 448-amino acid (a.a. 28-475) enzyme containing a PLAT (Polycystin-1, Lipoxygenase, Alpha-Toxin) domain (a.a. 341-464) and a heparin-binding domain (a.a. 346-441).

特異性

Clone 4-1a binds human and bovine LPL, as well as GPIHBP1-bound LPL, but not human hepatic lipase. Clone 4-1a does not inhibit LPL catalytic activity or LPL heparin interaction, nor does clone 4-1a compete against triglyceride-rich lipoproteins (LRLs) for bining GPIHBP1-LPL complex (Bensadoun, A., et al. (2014). Biochim. Biophys. Acta. 1841(7):970-976).

免疫原

Epitope: Near the N-terminus of mature LPL.

Purified human plasma lipoprotein lipase/LPL.

アプリケーション

Research Category
細胞シグナル伝達

Research Sub Category
脂質代謝及びエネルギー調節

Anti-Lipoprotein Lipase/LPL Antibody, clone 4-1a is an antibody against Lipoprotein Lipase/LPL for use in Immunohistochemistry (Paraffin), Western Blotting, ELISA, Immunocytochemistry, Immunofluorescence.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected lipoprotein lipase/LPL immunoreactivity in human pancreas, human skeletal muscle, and rat heart tissue sections.
Western Blotting Analysis: A representative lot detected human plasma lipoprotein lipase (LPL) and bovine milk LPL by Western blotting. Clone 4-1a exhibited much reduced binding to murine or chicken LPL, and failed to detect human hepatic lipase (hHL) (Bensadoun, A., et al. (2014). Biochim. Biophys. Acta. 1841(7):970-976).
Western Blotting Analysis: A representative lot detected recombinant wild-type human LPL (rhLPL), as well as rhLPL with mutated Gln3 & Arg4. Clone 4-1a failed to react with rhLPL with mutated Ile8 or Asp9, nor rhLPL with either a.a. 5-15 or aa.16-25 deletion (Bensadoun, A., et al. (2014). Biochim. Biophys. Acta. 1841(7):970-976).
ELISA Analysis: A representative lot was employed as the capture antibody for the detection of human LPL by sandwich ELISA, using biotinylated clone 5D2 as the detcting antibody (Bensadoun, A., et al. (2014). Biochim. Biophys. Acta. 1841(7):970-976).
Immunocytochemistry Analysis: A representative lot detected the binding of human LPL to CHO and CHL cells transfected to express surface GPIHBP1, but not to mock transfected cells or cells transfected to express GPIHBP1 C65S mutant. Clone 4-1a does not compete against triglyceride-rich lipoproteins (TRLs) for binding the LPL-GPIHBP1 complex on cell surface (Bensadoun, A., et al. (2014). Biochim. Biophys. Acta. 1841(7):970-976).
Immunofluorescence Analysis: A representative lot detected hLPL immunoreactivity associated with GPIHBP1 on capillary endothelial cells in OCT-embedded, methanol/acetone-fixed frozen skeletal muscle sections from mice expressing muscle-specific human LPL transgene. Much lower immunoreactivity of the endogenous mLPL was detected in skeletal muscle sections from non-transgenic mice (Bensadoun, A., et al. (2014). Biochim. Biophys. Acta. 1841(7):970-976).
Note: Clone 4-1a exhibits much weaker affinity toward rat & murine LPL when compared with human LPL. Higher amount of sample loading (in Western blotting and ELISA) and/or high sensitivity detection method (e.g. HRP polymer or fluorescence detection in Western blotting and immunohistochemistry) is recommended when using this clone for detecting rat & murine LPL.

品質

Evaluated by Immunohistochemistry in mouse heart tissue.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected lipoprotein lipase/LPL immunoreactivity in mouse heart tissue sections.

ターゲットの説明

53.16 kDa (ProLPL) and 50.39 (Mature LPL) calculated. ~56 kDa observed (Bensadoun, A., et al. (2014). Biochim. Biophys. Acta. 1841(7):970-976).

物理的形状

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable