MABS1225

Anti-TXNIP Antibody, clone JY2

clone JY2, from mouse

• 別名: Thioredoxin-interacting protein, Thioredoxin-binding protein 2, Vitamin D3 up-regulated protein 1
• UNSPSCコード: 12352203
• eCl@ss: 32160702

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: JY2, monoclonal
• 化学種の反応性: human, mouse
• テクニック: western blot: suitable
• アイソタイプ: IgG1κ
• NCBIアクセッション番号: NP_006463.3
• UniProtアクセッション番号: Q9H3M7
• 輸送温度: ambient
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... TXNIP(10628)

詳細

詳細

Thioredoxin-interacting protein (UniProt Q9H3M7; also known as Thioredoxin-binding protein 2, Vitamin D3 up-regulated protein 1) is encoded by the TXNIP (also known as VDUP1) gene (Gene ID 10628) in human. Thioredoxin interacting protein (TXNIP) is an alpha-arrestin protein that acts as an oxidative stress mediator that binds thioredoxin active cysteine residue and suppresses its antioxidant activity. Interacts with COPS5 and restores COPS5-induced suppression of CDKN1B stability, blocking the COPS5-mediated translocation of CDKN1B from the nucleus to the cytoplasm. TXNIP over-expression results in increased cellular ROS level and oxidative cell death, while TXNIP suppression enhances cellular resistance to oxidative injury. TXNIP is also shown to inhibit cellular glucose uptake by downregulating plasma membrane localization of glucose transporter 1 (GLUT1). TXNIP is required for the maturation of natural killer cells. Its expression is markedly down-regulated in tumor cells and its levels are correlated with clinical stage of cancer. Mice with mutations or knockout of TXNIP gene are much more susceptible to carcinogenesis than wild-type mice, indicating its role in cancer suppression. Ref.: Masutani, H., et al. (2012). J. Clin. Biochem. Nutr. 50, 23-34.

特異性

Clone JY2 detected TXNIP in adult mouse cardiomyocytes. No TXNIP target band was detected in cardiomyocytes from Txnip-null mice (Yoshioka, J., et al. (2007). Circ. Res. 101(12):1328-1338).

免疫原

Recombinant full-length human TXNIP protein.

アプリケーション

Research Category
細胞シグナル伝達

Detect TXNIP/VDUP1 using this mouse monoclonal Anti-TXNIP Antibody, clone JY2, Cat. No. MABS1225, validated for use in Western Blotting.

Western Blotting Analysis: A representative lot detected full-length human TXNIP, but not human TXNIP constructs lacking C-terminal end a.a. 302-391 sequence, exogenously expressed in HEK293 cells (Yoshioka, J., et al. (2007). Circ. Res. 101(12):1328-1338).

Western Blotting Analysis: A representative lot detected TXNIP in adult mouse cardiomyocytes. No TXNIP target band was detected in cardiomyocytes from Txnip-null mice (Yoshioka, J., et al. (2007). Circ. Res. 101(12):1328-1338).

品質

Evaluated by Western Blotting in HeLa cells.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected TXNIP in 10 µg of HeLa cell lysate.

ターゲットの説明

~53 kDa observed. 43.66/37.32 kDa (human isoform 1/2) and 44.36/44.29 kDa (mouse isoform 1/2) calculated. Uncharacterized bands may be observed in some lysate(s).

物理的形状

Protein G purified.

Format: Purified

Purified mouse IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1